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4 protocols using d α tocopherol

1

Lipid-Based Nanoparticle Formulation

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Miglyol 812 N (medium chain triglycerides) was supplied from Sasol GmbH (Hamburg, Germany), and soya lecithin hydrogenated (Lipoid®S75) was obtained from Lipoid GmbH (Ludwigshafen am Rhein, Germany). Polysorbate 80 (Tween®80), penicillin, streptomycin, l-glutamine, Hank’s balanced salt solution (HBSS), and Alamar blue® were obtained from Invitrogen (Alfagene Portugal), and d-α-tocopherol was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultra-purified water was obtained from Milli® Q Plus system, home supplied.
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2

Analyzing Yeast Lipid Composition

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Commercially available dry baking yeast S. cerevisiae (Belbake dried yeast, Niemcy, Germany; batch L399854, according to the supplier stabilized with sorbitan monostearate) was purchased from a local store (LIDL, Cornigliano, Genova, Italy). Sodium dodecyl sulfate (SDS), ethanol, HPLC grade methanol, isopropyl alcohol, acetone, anhydrous sodium hydroxide, trifluoroacetic acid (Pour LC-MS), (R)-(+)-limonene, linalool, D-α-tocopherol, and 1,6-dihydrocarvone (mixture of isomers) were purchased from Sigma (Merck KGaA, Milan, Italy); sodium chloride and Nile Red were obtained from Fluorochem (Hadfield, UK), 2 N hydrochloric acid came from Alfa Aesar (Heysham, UK). Concanavalin A-Alexa Fluor 488 was supplied by Thermo Fisher Scientific (Wien, Austria).
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3

Antioxidant-rich oil and extract study

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DHA-rich oil (DHAO) made from deep-sea fish oil concentrate was kindly provided by Atrium innovations Inc. (Antwerpen, Belgium). Green tea leaf extract (GTE) containing 20% catechin was a product of Naturex (Avignon, France); D-α-tocopherol (1000 IU/g) was purchased from Sigma-Aldrich (Schnelldorf, Germany). All other chemicals used in this study were either of GC-grade, HPLC-grade or analytical grade.
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4

Radiolabeling of PSMA-617 with Gallium-68

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Sodium oleate, Tween 80, polyvinyl alcohol (PVA) (97%-98% hydrolysed and Mw: 13-23 kDa), lauric acid, polyethylene glycol (PEG) 4000, and d-α-tocopherol were purchased from Sigma Aldrich (St Louis, MO, USA). HPLC-grade ethanol was purchased from Radchem Products Inc. (Orlando Park, Il, USA). All chemicals, reagents, and solvents for the radiosynthesis (ie, ultrapure, metal-free water) and analysis of the compounds were at least of analytical grade or were purchased from Sigma Aldrich (St Louis, MO, USA). PSMA-617 (C49H71N9O16; MW = 1042.1 g/mol) was purchased from Advanced Biochemical Compounds (Radeberg, Germany). Figure 1 shows the chemical structure of this compound including the [ 68 Ga]Ga 3+ -complex with DOTA.
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