Primary antibodies used for immunohistochemistry were: Mouse anti-β-galactosidase (1:5000; ref 18637314, Promega), rabbit anti-TRPV1 (1:1000, ref sc-28759, Santa Cruz), rabbit anti-CGRP (1:1000, ref AB15360, Abcam), rabbit anti-substance P (1:500, ref 84500505, Biogenesis Ltd), rabbit anti-TrkA (1:500, ref SAB1305371, Sigma), goat anti-c-ret (1:500; ref AF482, R&D System), rabbit anti-phospho GluN1 (Ser896, 1:1000; Upstate), rabbit anti-phospho-Erk (1:1000, ref 9101, Cell Signalling), rabbit anti-HA (1:500, ref 715500, Life Technologies), mouse anti-Iba1 (1:2000, ref MNK4428, Wako), Griffonia simplicifolia isolectin (IB4) FITC conjugated (1:1000, Sigma). Secondary antibodies were Alexa Fluor 488 anti-rabbit or anti-goat antibody (1:2000) from Life Technologies, and Cy3-conjugated anti-mouse antibody (1:2000) was from Jackson ImmunoResearch Laboratories.
Mnk4428
Manufactured by Fujifilm
The MNK4428 is a lab equipment product from Fujifilm. It is designed for use in various laboratory settings. The core function of the MNK4428 is to provide precise and reliable measurements, but detailed specifications are not available.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β galactosidase, by Promega (1 mentions)
Cy3 conjugated anti mouse antibody, by Jackson ImmunoResearch (1 mentions)
Sc 28759, by Santa Cruz Biotechnology (1 mentions)
Ab15360, by Abcam (1 mentions)
T9026, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit a488, by Thermo Fisher Scientific (1 mentions)
Donkey anti goat hrp, by Jackson ImmunoResearch (1 mentions)
Anti ha beads, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions)
2 protocols using mnk4428
1
Immunohistochemistry Protocol for Neuronal Markers
2
Immunocytochemistry and Western Blot Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunocytochemistry goat anti-ApoE (1:1000, Millipore, AB947), goat anti-Cathepsin B (1:1000, R&D systems, AF965), rabbit anti-P2X4 (1:200, Sigma, HPA039494), donkey anti-CD68 (1:300, Biorad, MCA1957A488T), mouse anti-6E10 (1:500, Biolegend, SIG-39320–0200), rabbit anti-Iba1 (Ionized calcium-binding adapter molecule 1, 1:2000, Wako, MNK4428), rat anti-P2X4 (1:200, kindly provided by Dr. Nolte [31 (link)]), donkey anti-rat-A594 (1:500, Jackson Immunoresearch, 712-586-150), donkey anti-goat-A488 (1:2000, Molecular probes, A11055), donkey anti-rabbit-A557 (1:2000, R&D systems, NL004), goat anti-rabbit-A488 (1:2000, Molecular probes, A11034).
Western blot mouse anti-tubulin (1:5000, Sigma, T9026), rabbit anti-HA (1:500, Invitrogen, 715,500), rabbit anti-P2X4 (1:500, Alomone Labs, APR-002), anti-HA beads (1:500 Santa-Cruz Biotechnology), horse anti-mouse-HRP (1:2000, Cell signaling, 7076S), goat anti-rabbit-HRP (1:2000, Jackson Immunoresearch, 111-035-144), donkey anti-goat-HRP (1:2000, Jackson Immunoresearch, 705–035-003).
Western blot mouse anti-tubulin (1:5000, Sigma, T9026), rabbit anti-HA (1:500, Invitrogen, 715,500), rabbit anti-P2X4 (1:500, Alomone Labs, APR-002), anti-HA beads (1:500 Santa-Cruz Biotechnology), horse anti-mouse-HRP (1:2000, Cell signaling, 7076S), goat anti-rabbit-HRP (1:2000, Jackson Immunoresearch, 111-035-144), donkey anti-goat-HRP (1:2000, Jackson Immunoresearch, 705–035-003).
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