Recombinant human egf protein

PLSCR1 shRNA and STAT3 shRNA were purchased from Sigma-Aldrich (St Louis, MO). Human PLSCR1, STAT3, and EGFR were amplified from the MDA-MB231 cDNA and subcloned into pLenti6.3⁄V5, pLVX, and pCMV, respectively.
Recombinant human EGF protein, human interleukin-6 (hIL-6), and antibodies against PLSCR1 and ALDH1 were purchased from Abcam (Carlsbad, CA). Antibodies against STAT1, STAT3, Phospho- STAT1 (Tyr701) and Phospho-STAT3 (Tyr705) were acquired from Cell Signaling Technology (Danvers, MA). Antibodies against phospho-Tyr were obtained from Santa Cruz (Dallas, TX). 6x-His Tag monoclonal antibody and Alexa Fluor 555-conjugated goat anti-rabbit IgG were purchased from Thermo Fisher Scientific. Antibodies for Flag, Myc, β-Tubulin, LaminB and Anti-FLAG Magnetic Beads were obtained from Sigma-Aldrich (St. Louis, MO).

All reagents and antibodies adopted in this study were purchased from Chinese suppliers (in parentheses): POS (MCE, HY-17373), chloroquine (Sigma, C6628), acridine orange (Sigma, 158550), dimethyl sulfoxide (DMSO, Sigma, 67–68-5), Cell Counting Kit-8 (CCK-8, Dojindo, CK04), Cell Cycle Detection Kit (KeyGEN, KGA512), BrdU Cell Proliferation Assay Kit (Abcam, ab287841), recombinant human EGF protein (Abcam, ab9697), recombinant human FGF protein (Abcam, ab9596), B-27 (Gibco, 17504–044), N-2 (Gibco, 17502–048), CD133 (Abcam, ab19898), SOX2 (Abcam, ab97959), Nanog (Abcam, ab21624), Oct4 (Abcam, ab19857), Beclin1 (Abcam, ab62557), Atg5 (Abcam, ab108327), survivin (Abcam, ab76424), Wnt1 (Abcam, ab15251), β-catenin (Abcam, ab32572), c-Myc (Abcam, ab32072), CD44-FITC (Abcam, ab27285), CD133-APC (Abcam, ab253259), SQSTM1 (MBL, PM045) and LC3 (MBL, PM036).