Annexin 5 pe apoptosis detection kit

Manufactured by Abcam

Sourced in United States, United Kingdom

The Annexin V-PE apoptosis detection kit is a laboratory tool used to detect and analyze apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. The Annexin V is conjugated to the fluorescent dye phycoerythrin (PE), allowing for the visualization and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Sc 5261, by Cell Signaling Technology (2 mentions) Facscanto 2, by BD (2 mentions) Facsdiva 7, by BD (1 mentions) Facsverse flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V Apoptosis Detection Kit Annexin V apoptosis kit Annexin V Detection Kit Annexin V-PE Apoptosis Detection Kit Annexin V kit Annexin V

6 protocols using annexin 5 pe apoptosis detection kit

Apoptosis detection via immunoblotting

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The cell lysates were separated by SDS–PAGE and followed by immunoblotting using antibodies against HuR (Santa Cruz, sc-5261), caspase-3 (Cell Signaling, 8G10) and COX-2 (One World Lab, 43977). Apoptosis assay was carried out based on manufacturer’s protocol (Annexin V-PE apoptosis detection kit; Biovision, K128-100).

Janakiraman H., House R.P., Talwar S., Courtney S.M., Hazard E.S., Hardiman G., Mehrotra S., Howe P.H., Gangaraju V, & Palanisamy V. (2016). Repression of caspase-3 and RNA-binding protein HuR cleavage by cyclooxygenase-2 promotes drug resistance in oral squamous cell carcinoma. Oncogene, 36(22), 3137-3148.

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Apoptosis detection via immunoblotting

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Analyzing Apoptosis with Annexin V-PE

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The Annexin V-PE Apoptosis Detection kit (Biovision, K128, US) was used to analyze apoptosis, according to the manufacturer's instructions. Cisplatin (10 μM) was used as a positive control. Cells were analyzed by flow cytometry (BD, FacsCanto II) and results were analyzed with BD CellQuestPro software.

Gunes A., Bagirsakci E., Iscan E., Cakan-Akdogan G., Aykutlu U., Senturk S., Ozhan G., Erdal E., Nart D., Barbet F.Y, & Atabey N. (2018). Thioredoxin interacting protein promotes invasion in hepatocellular carcinoma. Oncotarget, 9(96), 36849-36866.

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Annexin V Apoptosis Assay for M231 Cells

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Annexin V apoptosis assay was carried out following the instructions of Annexin V-PE apoptosis detection kit (Abcam, Cat#ab14155). Briefly, 100,000 cells (M231 control cells or NR4A1-null cells) collected from the fresh culture (cell confluency no more than 80%) were first washed with PBS and resuspended in 1x binding buffer, 5 μl Annexin V-PE probe together with DAPI (1:5,000 diluted) were added into the cell suspension and cells were incubated for 5–10 minutes before flow cytometry analysis. Dead cells or apoptotic cells were stained positive for both Annexin V-PE and DAPI.

Guo H., Golczer G., Wittner B.S., Langenbucher A., Zachariah M., Dubash T.D., Hong X., Comaills V., Burr R., Ebright R.Y., Horwitz E., Vuille J.A., Hajizadeh S., Wiley D.F., Reeves B.A., Zhang J.M., Niederhoffer K.L., Lu C., Wesley B., Ho U., Nieman L.T., Toner M., Vasudevan S., Zou L., Mostoslavsky R., Maheswaran S., Lawrence M.S, & Haber D.A. (2021). NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer. Molecular cell, 81(19), 4041-4058.e15.

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Annexin V-PE Apoptosis Detection

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Apoptosis was measured by the Annexin V-PE Apoptosis Detection Kit (Abcam, Cambridge, MA). FACS was run in FACSCanto™ II (BD Biosciences, San Jose, CA) and data were analyzed by FACSDiva 7 software (BD Biosciences).

Lee K.S., Park J.L., Lee K., Richardson L.E., Johnson B.H., Lee H.S., Lee J.S., Kim S.B., Kwon O.H., Song K.S., Kim Y.S., Ashktorab H., Smoot D.T., Jeon S.H., Kim S.Y, & Lee Y.S. (2014). nc886, a non-coding RNA of anti-proliferative role, is suppressed by CpG DNA methylation in human gastric cancer. Oncotarget, 5(11), 3944-3955.

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Apoptosis Assay in SH-SY5Y Cells

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SH-SY5Y cells (5 × 10⁵ cells) were harvested and then stained using an Annexin V-PE apoptosis detection kit (Abcam, Cambridge, UK) to assess the proportion of apoptotic cells. The stained cells were analyzed by a FACSVerse flow cytometer (BD, NJ, USA) and FlowJo V10 software (FlowJo, OR, USA). The experiments were conducted under the same experimental conditions with 20,000 cells per group.

Park J.S., Choe K., Lee H.J., Park T.J, & Kim M.O. (2023). Neuroprotective effects of osmotin in Parkinson’s disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways. Journal of Biomedical Science, 30, 66.

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