Log In Sign up
The largest database of trusted experimental protocols

Recombinant il 1α

Manufactured by R&D Systems
Visit Supplier

Recombinant IL-1α is a laboratory reagent produced through recombinant DNA technology. It is an interleukin-1 alpha protein with the primary function of stimulating immune responses.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant human tgf β1, by Cell Signaling Technology (2 mentions) Wi 38, by American Type Culture Collection (2 mentions) Gw788388, by Bio-Techne (2 mentions) Recombinant human tgf β2, by Thermo Fisher Scientific (2 mentions) Htert rpe1 cells, by American Type Culture Collection (2 mentions) Recombinant human tgf β3, by Thermo Fisher Scientific (2 mentions) 4 hydroxytamoxifen 4 oht, by Merck Group (2 mentions) Sb431542, by Bio-Techne (2 mentions) Hacat cells, by American Type Culture Collection (2 mentions) Imr 90, by American Type Culture Collection (2 mentions) A83 01, by Bio-Techne (2 mentions) Etoposide, by Merck Group (2 mentions) Neutrophil elastase, by R&D Systems (1 mentions) Budesonide, by Thermo Fisher Scientific (1 mentions) Il 36ra, by R&D Systems (1 mentions) Proteinase 3, by R&D Systems (1 mentions) Il 36γ, by R&D Systems (1 mentions) Il 36β, by R&D Systems (1 mentions) Il 36α, by R&D Systems (1 mentions) Poly 1 c, by Merck Group (1 mentions)

Close spelling variants of this product

IL-1α (97 citations) Recombinant human IL-1α (9 citations) Recombinant mouse IL-1α (5 citations) Recombinant human IL-1α or IL-1β (4 citations) Recombinant IL-1α proteins (2 citations)

4 protocols using recombinant il 1α

1

Fibroblast Culture and Pharmacological Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols
IMR90 (ATCC), WI38 (ATCC) and ESFs (embryonic skin fibroblasts)49 (link) (a kind gift from Dr. Jesus Gil, Imperial College, London) human diploid fibroblasts were cultured as previously described in DMEM /10% fetal calf serum (FCS) in a 5% O2 / 5% CO2 atmosphere. hTERT-RPE1 cells (a telomerase-immortalised human retinal pigment epithelial cell line) (ATCC) were grown in DMEM/F12 / 10% FCS in a 5% O2 / 5% CO2 atmosphere. HACAT, cells (ATCC) were cultured in DMEM / 10% FCS in a 21% O2 / 5% CO2 atmosphere. No cell lines used in this study were found in the database of commonly misidentified cell lines that is maintained by ICLAC and NCBI Biosample. Cell identity was confirmed through STR genotyping. Regular testing was always negative for mycoplasma contamination.
The following drugs and inhibitors were used: 4-hydroxytamoxifen (4OHT) (Sigma); N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester (DAPT) (Sigma); SB431542 (Tocris); A 83-01 (Tocris); GW788388 (Tocris); Etoposide (Sigma); recombinant human TGF-β1 (Cell Signaling); recombinant human TGF-β2 (Peprotech); recombinant human TGF-β3 (Peprotech); Tumor necrosis factor alpha (TNF-α); recombinant IL-1α (both R&D systems).
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
+ Open protocol
+ Expand
2

Inflammatory Cytokine and Cigarette Smoke Exposure Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant IL-1α, IL-36α, IL-36β, IL-36γ, IL-36Ra, TNF-α, neutrophil elastase, cathepsin G, proteinase-3, and anti–IL-36γ antibody (catalog AF2320) were purchased from R&D Systems. Rabbit anti–goat Ig/HRP (P0449) was purchased from Agilent. Poly(I:C) was purchased from Sigma-Aldrich. Nontypeable H. influenzae were obtained from the National Collection of Type Cultures (strain no. 1269) and were heat-killed by incubation at 65°C for 10 minutes as described previously (55 (link)). CSE was generated as previously described (56 (link)) from full-strength Marlboro cigarette (Phillip Morris). Budesonide was purchased from Thermo Fisher Scientific.
Baker J.R., Fenwick P.S., Koss C.K., Owles H.B., Elkin S.L., Fine J., Thomas M., El Kasmi K.C., Barnes P.J, & Donnelly L.E. (2022). IL-36 receptor agonist and antagonist imbalance drives neutrophilic inflammation in COPD. JCI Insight, 7(15), e155581.
+ Open protocol
+ Expand
3

Peanut Allergy Protein Extraction

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Peanut flour was purchased from the Golden Peanut Company (Alpharetta, Ga), endotoxin was undetectable (<0.5 EU/mg flour) as previously described (7 (link)). Crude peanut extract (7 (link)) and Alternaria alternata extract (19 (link)) were purchased from Greer Laboratories (Lenoir, NC). Recombinant IL-1α was purchased from R&D Systems (Minneapolis, MN), and recombinant IL-13 was purchased from Biolegend (San Diego, Ca).
Krempski J.W., Kobayashi T., Iijima K., McKenzie A.N, & Kita H. (2020). Group 2 Innate Lymphoid Cells Promote Development of T follicular Helper (Tfh) Cells and Initiate Allergic Sensitization to Peanuts. Journal of immunology (Baltimore, Md. : 1950), 204(12), 3086-3096.
+ Open protocol
+ Expand
4

Fibroblast Culture and Pharmacological Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols
IMR90 (ATCC), WI38 (ATCC) and ESFs (embryonic skin fibroblasts)49 (link) (a kind gift from Dr. Jesus Gil, Imperial College, London) human diploid fibroblasts were cultured as previously described in DMEM /10% fetal calf serum (FCS) in a 5% O2 / 5% CO2 atmosphere. hTERT-RPE1 cells (a telomerase-immortalised human retinal pigment epithelial cell line) (ATCC) were grown in DMEM/F12 / 10% FCS in a 5% O2 / 5% CO2 atmosphere. HACAT, cells (ATCC) were cultured in DMEM / 10% FCS in a 21% O2 / 5% CO2 atmosphere. No cell lines used in this study were found in the database of commonly misidentified cell lines that is maintained by ICLAC and NCBI Biosample. Cell identity was confirmed through STR genotyping. Regular testing was always negative for mycoplasma contamination.
The following drugs and inhibitors were used: 4-hydroxytamoxifen (4OHT) (Sigma); N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester (DAPT) (Sigma); SB431542 (Tocris); A 83-01 (Tocris); GW788388 (Tocris); Etoposide (Sigma); recombinant human TGF-β1 (Cell Signaling); recombinant human TGF-β2 (Peprotech); recombinant human TGF-β3 (Peprotech); Tumor necrosis factor alpha (TNF-α); recombinant IL-1α (both R&D systems).
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!