From each sample of the diet, an aliquot (10 g) was homogenized with 90 mL of sterile saline solution (PBS). The aliquots of the mouse feces (100 mg) were homogenized with 0.9 mL of PBS. Subsequently, a decimal dilution series prepared in PBS and 500 μL of the appropriate dilutions were plated on Dextrose Casein-peptone agar (Merck, Darmstadt, Germany). Removal of vegetative forms of bacteria was carried out by heating the homogenate for 15 min at 85 °C. The plates were incubated aerobically at 36 °C for 48 h.
Dextrose casein peptone agar
Manufactured by Merck Group
Sourced in Germany
Dextrose Casein-peptone agar is a microbiological culture medium used for the cultivation and enumeration of microorganisms. It contains dextrose, casein peptone, and agar as the primary components, providing a nutrient-rich environment for the growth of a wide range of bacteria and other microbes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dextrose casein peptone agar
1
Quantification of Bacterial Spores in Mouse Feces
2
Spore-forming Bacillus subtilis 20 Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The kanamycin-resistant strain Bacillus subtilis 20 was used to feed the animals. Strain selection for the experiment was substantiated by its ability to grow on selective kanamycin-added medium that allows for precisely calculating CFU for that strain. Bacteria were cultured on plates of Dextrose Casein–peptone agar (Merck, Darmstadt, Germany) at +37 °C for 72–96 h until complete spore formation. Spores of the bacteria were resuspended in sterile phosphate-buffered saline (PBS) solution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!