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Baf410

Manufactured by R&D Systems

BAF410 is a laboratory instrument designed for the detection and analysis of cellular proteins. It utilizes a combination of fluorescent labeling and flow cytometry techniques to quantify the expression levels of specific proteins within individual cells. The core function of the BAF410 is to provide researchers with a reliable and efficient method for studying protein expression patterns in a variety of cell types and experimental conditions.

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2 protocols using baf410

1

ELISA for Mouse IL-6 and TNF-α

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ELISA analyses for IL-6 and TNF-α were performed as previously described [25 (link)]. Antibodies used were: anti-mouse IL-6 (MP5-20 F3, Biolegend) 0.5 μg/mL; biotin anti-mouse IL-6 (MP5-32C11, Biolegend) 1 μg/mL; anti-mouse TNF-α (R&D systems, AF-410-NA) 0.5 μg/mL; biotin anti-mouse TNF-α (R&D systems, BAF410) 0.25 μg/mL.
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2

Cytokine Detection by ELISA

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ELISA for IL-6 and TNF-α were performed as previously described (25 (link)). Antibodies used were: anti-mouse IL-6 MP5–20F3 (Biolegend, 504501) 0.5μg/mL; biotin anti-mouse IL-6 MP5–32C11 (Biolegend, 504601) 1μg/mL; anti-mouse TNF-α (R&D systems, AF-410-NA) 0.5μg/mL; biotin anti-mouse TNF-α (R&D systems, BAF410) 0.25μg/mL.
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