Bone marrow cells were flushed from femora and tibiae into HBSS containing 2% FCS (Himedia, Mumbai, India) using a 21-gauge needle and syringe. Cells were centrifuged in Histopaque-1083 to isolate BM-MNCs. Cell were cultured in RPMI-1640 containing 10% fetal bovine serum, 2 mm glutamine, 10 μm HEPES buffer, 100 units per ml penicillin, and 100 μg ml−1 streptomycin (Himedia, Mumbai, India) at density of 1 × 106 cells per ml.
Hanks balanced salt solution (hbss)
Manufactured by HiMedia
Sourced in India
HBSS is a balanced salt solution designed for use in cell and tissue culture applications. It provides a physiologically balanced environment for the maintenance and growth of cells in vitro. The solution contains a combination of salts, glucose, and other components to support cellular function and viability.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by HiMedia (1 mentions)
Streptomycin, by HiMedia (1 mentions)
Rpmi 1640, by HiMedia (1 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Collagenase type 4, by HiMedia (1 mentions)
Transfer buffer, by Genetix (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ponceau s, by HiMedia (1 mentions)
Jc 1 assay kit, by Thermo Fisher Scientific (1 mentions)
Protein assay kit, by Avantor (1 mentions)
Cdna synthesis kit, by Genetix (1 mentions)
Ripa lysis buffer, by Avantor (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Merck Group (1 mentions)
Sodium cacodylate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hematoxylin, by HiMedia (1 mentions)
3 protocols using hanks balanced salt solution (hbss)
1
Isolation and Culture of Bone Marrow Mononuclear Cells
2
Evaluating Cytotoxicity and Apoptosis in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ALA (463-40-1) (0.914gm/ml) was purchased from TCI Chemicals (India) Pvt. Ltd. RPMI 1640 (Gibco, USA); fetal bovine serum (FBS) (Gibco-10270); trypsin (Gibco, USA); eagle balanced salt solution (EBSS)(Gibco, 2018-11); hank’s balanced salt solution (HBSS) (Himedia, TL1190); EtBr (Himedia, MB071); AO(Himedia, MB116); JC-1 assay kit (Thermo Scientific, M34152); PI (SC-3541); tamoxifen citrate (TMX) (Tammodex 20, Biochem Pharmaceuticals India); penicillin- streptomycin (Thermo Scientific, 15410-163); gentamycin (Thermo Scientific, 15710-049);3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) (Himedia, TC191); RNase (SRL, 58895); dimethyl sulfoxide (DMSO) (Merck, 1.16743.0521); DMBA (Sigma Aldrich, 57-97-6); ponceau S (Himedia, ML045); sodium cacodylate (Sigma Aldrich, C0250); collagenase type 4 (Himedia, TC-214); hyaluronidase (Himedia, TC331); hematoxylin (Himedia, S058); eosin (Himedia, S007); RIPA lysis buffer (Amresco, N653); protein assay kit (Amresco, M173); bovine serum albumin (BSA) (Genetix, PG-2330); transfer buffer (Genetix, GX-9411AR), trizol reagent (Sigma-T9424), cDNA synthesis kit (Genetix-K1612). Caspase 3 (SC-4263) and caspase 8 (SC-4267) assay kits were procured from Santacruz Biotechnology Inc., California, Delaware. All others chemicals were of molecular biology grade and purchased from Genetix Biotech Asia Pvt. Ltd, New Delhi.
3
Culturing SK-N-SH Neuroblastoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SK-N-SH neuroblastoma cells were grown in Dulbecco's Modified Eagle's Medium (DMEM; GIBCO/Invitrogen Life Technologies, Paisley, UK) with 10% fetal bovine serum (FBS; JRH, Woodland, CA, USA). Cells were maintained at 37 °C in an incubator within an atmosphere of 5% carbon dioxide (CO2). Cells were routinely passaged using trypsin (0.25%)–EDTA (0.1%) solution in Hank's Balanced Salt Solution (HBSS; Himedia Laboratories Pvt. Ltd., Mumbai, India).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!