Blood sample (2.5 ml) was collected in a PAXgene RNA blood collection tube (Becton-Dickinson, Mountain View, California, USA) and used for gene expression analysis. Another blood sample (2 ml) was collected in a blood collection tube containing ethylenediaminetetraacetic acid-2K and used for flow cytometry, blood count, differential leukocyte count, and lymphocyte subpopulation cell count. Another blood sample (8 ml) was collected in a blood collection tube for mononuclear cell separation (Becton–Dickinson) and used for the isolation of mononuclear cells for immunological analysis. Separately, blood biochemical tests were performed for screening (HbA1c) and safety checking.
Paxgene rna blood collection tube
Manufactured by BD
Sourced in United States
The PAXgene RNA blood collection tube is a pre-evacuated plastic tube designed for the collection, stabilization, and transportation of blood samples for RNA analysis. The tube contains reagents that immediately stabilize RNA molecules upon blood collection, preserving the RNA profile of the sample.
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Lab products found in correlation
2 protocols using paxgene rna blood collection tube
1
Blood Sample Collection for Molecular and Immunological Analysis
2
PAXgene RNA Extraction from Blood
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Blood for RNA extraction was collected using a PAXgene® RNA blood collection tube (Becton, Dickinson and Company, NJ, USA). After mixing, the mixture was allowed to stand at room temperature (20-21 °C) for 2 h before being frozen at -20 °C, and total RNA was extracted using the PAXgene® blood miRNA kit (PreAnalytiX GmbH, Hombrechtikon, Switzerland).
The concentration and quality of the extracted total RNA was measured using a bioanalyzer (2100 Bioanalyzer, Agilent Technologies, Inc., CA, USA) (RNA Integrity Number 7.7-9.6, average 8.6). RNA was stored at -80 °C until use.
The concentration and quality of the extracted total RNA was measured using a bioanalyzer (2100 Bioanalyzer, Agilent Technologies, Inc., CA, USA) (RNA Integrity Number 7.7-9.6, average 8.6). RNA was stored at -80 °C until use.
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