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3 protocols using cerium nitrate ce no3 3 6h2o

1

Paracetamol-Chitosan-Cerium Nitrate Formulation

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Paracetamol, chitosan and cerium nitrate (Ce(NO3)3·6H2O) were obtained from Sigma-Aldrich Chemicals (St. Louis, Mo, USA). Zinc acetate (Zn(C2H3O2)2·2H2O), glassy carbon microspheres (GCMs, particle size 0.4–12 micron), 4-aminophenol and paraffin oil were obtained from Alfa Aesar (Ward Hill, MA). Standard stock solutions were prepared separately by dissolving Paracetamol and p-aminophenol in anhydrous ethanol and kept in darkness at 4 °C. Phosphate buffer solutions (PBS, 0.2 M) of pH 2.0–8.0 were prepared from orthophosphoric acid and its salts and used as a supporting electrolyte in the current study. All aqueous solutions were freshly prepared with doubly-distilled water from a Millipore system (Milli-pore Inc., 18.2 MΩ cm).
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2

Bioactive Calcium Phosphate Composite

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For the development of the research, the following reagents were used: Calcium hydroxide—Ca(OH)2 (ENSURE®, Duque de Caxias, Rio de Janeiro, Brazil); dibasic ammonium phosphate—(NH4)2HPO4 (Sigma-Aldrich, St. Louis, MI, USA); and cerium nitrate—Ce(NO3)3·6H2O (Sigma-Aldrich); sodium hydroxide—NaOH (ISOFAR, Duque de Caxias, Rio de Janeiro, Brazil); nutrient mixture F-12 (DMEM/F-12) (Sigma-Aldrich); 3-4,5 dimethylthiazol-2,5-diphenyl tetrazolium bromide (MTT) (Sigma-Aldrich); dimethylsulfoxide (DMSO) (Mallinckrodt Chemicals, Phillipsburg, NJ, USA); cashew gum isolated; deionized water and gellan gum, kindly provided by KelcoGel®, Limeira, São Paulo, Brazil.
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3

Synthesis and Characterization of ZnO and CeO2

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For the seeding of ZnO, zinc acetate and sodium hydroxide (NaOH) were supplied from lobacheme. For the production of CeO2, cerium nitrate Ce (NO3)3.6H2O was bought from Sigma Aldrich. Merck provided the Dimethylsulfoxide (DMSO) and nutrient agar media that were utilized to determine Zone of Inhibition (ZOI). All compounds were analytical grade and require no further purification.
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