Nuclear extracts were prepared from hippocampal cells using a buffer that contains 420 mM NaCl, 25% glycerol, 0.2 mM EDTA, 1 mM DTT, 20 mM HEPES pH 7.9, 1.5 mM MgCl2, and protease inhibitors as reported previously (28 (link), 58 (link)). Proteins were detected by western blot using specific antibodies: monoclonal Ezh2 (AC22, Cell Signaling), polyclonal Ezh1 (ab13665, Abcam), monoclonal JBP1/PRMT5 (611539, BD Transduction Laboratories), monoclonal Wdr5 (ab56919, Abcam), polyclonal RNA Pol II (sc-899, Santa Cruz Biotechnology), polyclonal TFIIB (sc-225, Santa Cruz Biotechnology).
Ab13665
Manufactured by Abcam
Ab13665 is a primary antibody product from Abcam. It is a monoclonal antibody that targets a specific antigen. This product is intended for use in various research applications.
Automatically generated - may contain errors
2 protocols using ab13665
1
Nuclear Protein Extraction and Western Blotting
2
Western Blot Analysis of PRC2 Complex
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