Akta 900 fast protein liquid chromatography

Manufactured by GE Healthcare

The AKTA 900 is a fast protein liquid chromatography (FPLC) system designed for efficient and reliable protein purification. It is a high-performance instrument capable of high-throughput and high-resolution separation of biomolecules. The AKTA 900 utilizes advanced fluidics and control systems to enable precise and reproducible separations across a wide range of applications.

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Lab products found in correlation

Bl21 codonplus de3 cells, by Thermo Fisher Scientific (2 mentions) Hitrap, by Cytiva (2 mentions) Hi trap affinity columns, by GE Healthcare (1 mentions) Gst trap hp columns, by GE Healthcare (1 mentions)

Spelling variants of this product

Fast protein liquid chromatography (14 citations) AKTA Fast Protein Liquid Chromatography (2 citations)

2 protocols using akta 900 fast protein liquid chromatography

Purification and Antibody Generation for Drosophila Rab11

Cited 2 times

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GST-full-length Drosophila Rab11 was expressed in BL21-CodonPlus [DE3] cells (Invitrogen) and purified using HiTrap affinity columns (GSTtrap FF, and GSTtrap HP columns, GE Healthcare) operated with AKTA 900 Fast Protein Liquid Chromatography as previously described [13 (link)]. Polyclonal antisera were raised against the purified GST-Rab11 protein. Polyclonal anti-anillin antibodies were raised against the N-terminal 270 amino acids of anillin, following the procedure described in [69 (link)]. Immunization was carried out at Agro-Bio Services (www.agro-bio.com) using standard procedures. The anti-GST-Rab11 and anti-anillin antisera were depleted of anti-GST antibodies and affinity-purified against either GST-Rab11 or GST-anillin before use in immunoblotting.

Giansanti M.G., Vanderleest T.E., Jewett C.E., Sechi S., Frappaolo A., Fabian L., Robinett C.C., Brill J.A., Loerke D., Fuller M.T, & Blankenship J.T. (2015). Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila. PLoS Genetics, 11(11), e1005632.

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Purification and Antibody Production of Drosophila GOLPH3

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GST-full-length Drosophila GOLPH3 (dGOLPH3) proteins and GST-full-length Drosophila Rab11 were expressed in BL21-CodonPlus [DE3] cells (Invitrogen) and purified using HiTrap affinity columns (GTtrap FF, and GSTtrap HP columns, GE Healthcare) operated with AKTA 900 Fast Protein Liquid Chromatography. Polyclonal antisera were raised against the purified GST-dGOLPH3 protein. Immunization was carried out at Agro-Bio Services (www.agro-bio.com); two rabbits and two mice were injected using standard procedures. The anti-GST-GOLPH3 antisera were first depleted of anti-GST antibodies and then affinity-purified against GST-GOLPH3.

Sechi S., Colotti G., Belloni G., Mattei V., Frappaolo A., Raffa G.D., Fuller M.T, & Giansanti M.G. (2014). GOLPH3 Is Essential for Contractile Ring Formation and Rab11 Localization to the Cleavage Site during Cytokinesis in Drosophila melanogaster. PLoS Genetics, 10(5), e1004305.

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