Oxford ct 3500 cryo holder

Manufactured by Philips

The Oxford CT-3500 cryo-holder is a device designed for use in electron microscopy. It is used to maintain a cryogenic environment for the specimen being observed, allowing for the study of sensitive samples at low temperatures.

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Lab products found in correlation

Talos 200c, by Thermo Fisher Scientific (3 mentions) Cm 120, by Ametek (3 mentions) Multiscan 791, by Philips (2 mentions) Cm120, by Thermo Fisher Scientific (1 mentions) Cryo holder, by Thermo Fisher Scientific (1 mentions) Us1000, by Ametek (1 mentions)

3 protocols using oxford ct 3500 cryo holder

Cryo-TEM Imaging of Nanoparticle Samples

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Samples were prepared to be at a mass percentage of 1% particles in PBS and the Cryo-TEM specimens were prepared in a controlled environment vitrification system (CEVS). Cryogenic transmission electron microscopy (cryo-TEM) imaging was performed either by a Phillips CM120 or a FEI Talos 200 C, FEG-equipped cryo-dedicated high-resolution transmission electron microscope (TEM and STEM), operated at an accelerating voltage of 120 kV. Specimens were transferred into an Oxford CT-3500 cryo-holder (Philips) or a Gatan 626DH (FEI) cryo-holder, and equilibrated below −178 °C. Specimens were examined using a low-dose imaging procedure to minimize electron-beam radiation damage. Images were recorded digitally by a Gatan Multiscan 791 cooled CCD camera (Philips CM 120), or a Gatan US 1000 high-resolution CCD camera (Tecnai T12 G2), using DigitalMicrograph software.

Soll M., Goswami T.K., Chen Q.C., Saltsman I., Teo R.D., Shahgholi M., Lim P., Di Bilio A.J., Cohen S., Termini J., Gray H.B, & Gross Z. (2019). Cell-Penetrating Protein/Corrole Nanoparticles. Scientific Reports, 9, 2294.

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Cryo-TEM Imaging of Nanoparticles

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Samples of NPs were prepared to be at a mass percentage of 1% particles in PBS and the Cryo-TEM specimens were prepared in a controlled environment vitrification system (CEVS). Cryogenic transmission electron microscopy (Cryo-TEM) imaging was performed using a FEI Talos 200C, FEG-equipped Cryo-dedicated high-resolution transmission electron microscope (TEM and STEM), operated at an accelerating voltage of 120 kV. Specimens were transferred into an Oxford CT-3500 Cryo-holder (Philips) or a Gatan 626DH (FEI) Cryo-holder, and equilibrated below −178 °C. Specimens were examined using a low-dose imaging procedure to minimize electron-beam radiation damage. Images were recorded digitally by a Gatan Multiscan 791 cooled CCD camera (Philips CM 120), or a Gatan US 1000 high-resolution CCD camera (Tecnai T12 G2), using DigitalMicrograph software.

Soll M., Chen Q.C., Zhitomirsky B., Lim P.P., Termini J., Gray H.B., Assaraf Y.G, & Gross Z. (2020). Protein-coated corrole nanoparticles for the treatment of prostate cancer cells. Cell Death Discovery, 6, 67.

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Cryo-TEM Imaging of Nanoparticle Suspensions

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Samples of NPs were prepared at a
mass percentage of 1% particles in PBS, and the cryo-TEM specimens
were prepared in a controlled environment vitrification system (CEVS).
Cryogenic transmission electron microscopy (cryo-TEM) imaging was
performed using an FEI Talos 200C, FEG-equipped cryo-dedicated high-resolution
transmission electron microscope (TEM and STEM), operated at an accelerating
voltage of 120 kV. Specimens were transferred into an Oxford CT-3500
Cryo-holder (Philips) or a Gatan 626DH (FEI) cryo-holder and equilibrated
below −178 °C. Specimens were examined using a low-dose
imaging procedure to minimize electron-beam radiation damage. Images
were recorded digitally by a Gatan Multiscan 791 cooled CCD camera
(Philips CM 120) or a Gatan US 1000 high-resolution CCD camera (Tecnai
T12 G2), using DigitalMicrograph software.

Soll M., Sharma V.K., Khoury S., Assaraf Y.G, & Gross Z. (2022). Corrole Nanoparticles for Chemotherapy of Castration-Resistant Prostate Cancer and as Sonodynamic Agents for Pancreatic Cancer Treatment. Journal of Medicinal Chemistry, 66(1), 766-776.

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