Chemicals for QD synthesis and silica overcoating such as trioctylphosphine oxide (TOPO), trioctylphosphine, cadmium oxide (CdO), selenium powder, dodecylamine, igepal and aminopropyl silane (APS) were purchased from Sigma–Aldrich and tetradecylphosphonic acid (TDPA) from Alfa Aesar and used as such without further purification.
Aminopropylsilane
Manufactured by Merck Group
Aminopropylsilane is a chemical compound used as a coupling agent and surface modifier in various laboratory applications. It is a colorless, odorless liquid that is commonly employed in the functionalization of silica-based materials, such as glass or silica gel. Aminopropylsilane is known for its ability to create a stable, covalent bond between organic and inorganic materials, making it a valuable tool in fields like chromatography, solid-phase extraction, and surface analysis.
Automatically generated - may contain errors
Lab products found in correlation
Glutaraldehyde, by Electron Microscopy Sciences (3 mentions)
Sulfo sanpah, by Thermo Fisher Scientific (3 mentions)
Ammonium persulfate, by Thermo Fisher Scientific (3 mentions)
Fibronectin, by Merck Group (2 mentions)
Dodecylamine, by Merck Group (1 mentions)
Trioctylphosphine, by Merck Group (1 mentions)
Selenium powder, by Merck Group (1 mentions)
Trioctylphosphine oxide, by Merck Group (1 mentions)
Igepal, by Merck Group (1 mentions)
Cadmium oxide, by Merck Group (1 mentions)
4 protocols using aminopropylsilane
1
Synthesis and Overcoating of Quantum Dots
2
Traction Force Microscopy on Polyacrylamide Gels
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Traction Force Microscopy (TFM) experiments were carried out on polyacrylamide (PA) gels that are polymerized onto 25 mm diameter (#1.5, Dow Corning) coverslips. Briefly, the coverslips are treated with a combination of aminopropylsilane (Sigma Aldrich) and glutaraldehyde (Electron Microscopy Sciences) to make the surface reactive to the acrylamide. The ratios of PA to bis-acrylamide for the gels used in this study are 4.3 kPa and 8.6 kPa. A concentration of 0.05% wt/vol ammonium persulfate (Fisher BioReagents) and 20 nM beads (Molecular Probes) are embedded in the gel mixture before polymerization. A 15 µl volume of the gel is added to the coverslip and covered with another coverslip, which has been made hydrophobic through treatment with Rain-X. The gels are polymerized on the coverslips for 30 min at room temperature. The gels are then reacted with the standard 1 mg/ml Sulfo-SANPAH (Thermo Fisher Scientific). The surface of the gels is then coated with fibronectin (F002, Sigma-Aldrich) with 0.5–1 mg/ml concentration. The reaction proceeds for 12 h overnight incubation in the dark, and the coverslips are then rinsed and stored in 1× PBS.
3
Traction Force Microscopy on Polyacrylamide Gels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Traction force Microscopy experiments were carried out on polyacrylamide (PA) gels, polymerized onto 25 mm diameter (#1.5, Dow Corning) coverslips. Briefly, the coverslips are treated with a combination of aminopropylsilane (Sigma Aldrich) and glutaraldehyde (Electron Microscopy Sciences) to make the surface reactive to the acrylamide. The ratios of polyacrylamide to bis-acrylamide for the gels is used as 7.5 % : 0.153 % to yield a gel with an elastic modulus of E= 4.3 kPa. A concentration of 0.05 % w/v ammonium persulfate (Fisher BioReagents) and 20 nM beads (Molecular Probes) of 0.1 µm size are embedded in the gel mixture prior to polymerization. A 15 µl of the gel is added to the coverslip and covered with another coverslip, which has been made hydrophobic through treatment with Rain-X®. The gels are polymerized on the coverslips for 30 minutes at room temperature. The gels are then reacted with the standard 1 mg/mL Sulfo-SANPAH (Thermo Fisher Scientific). The surface of the gels is then coated with fibronectin (F002, Sigma-Aldrich) with 0.5 mg/ml concentration. The reaction proceeds for 12 hours overnight incubation in the dark, and the coverslips are then rinsed and stored in 1X PBS.
4
Traction Force Microscopy on Polyacrylamide Gels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Traction force Microscopy experiments were carried out on polyacrylamide (PA) gels, polymerized onto 25 mm diameter (#1.5, Dow Corning) coverslips. Briefly, the coverslips are treated with a combination of aminopropylsilane (Sigma Aldrich) and glutaraldehyde (Electron Microscopy Sciences) to make the surface reactive to the acrylamide. The ratios of polyacrylamide to bis-acrylamide for the gels is used as 7.5 % : 0.153 % to yield a gel with an elastic modulus of E= 4.3 kPa. A concentration of 0.05 % w/v ammonium persulfate (Fisher BioReagents) and 20 nM beads (Molecular Probes) of 0.1 µm size are embedded in the gel mixture prior to polymerization. A 15 µl of the gel is added to the coverslip and covered with another coverslip, which has been made hydrophobic through treatment with Rain-X®. The gels are polymerized on the coverslips for 30 minutes at room temperature. The gels are then reacted with the standard 1 mg/mL Sulfo-SANPAH (Thermo Fisher Scientific). The surface of the gels is then coated with fibronectin (F002, Sigma-Aldrich) with 0.5 mg/ml concentration. The reaction proceeds for 12 hours overnight incubation in the dark, and the coverslips are then rinsed and stored in 1X PBS.
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