In total, 12 mice were chosen randomly for HepG2 cell inoculation at different time points. When the subcutaneous tumours grew to various sizes (0.06–1.0 g), the mice were sacrificed by humane euthanasia, and the tumours were excised, fixed in 10% formalin, embedded in paraffin, cut into slices (6-μm-thick) and dried. After dewaxing in xylene and hydrating in gradient ethyl alcohol (from 100 to 80%), the sections were incubated with the mouse anti-integrin αvβ3 monoclonal antibody (1:100, Abcam, Cambridge, USA), followed by sequential incubation with reagent A (reaction enhancer) and B (enzyme-conjugated goat anti-mouse/rabbit IgG polymer) of an immunohistochemical EliVision plus kit, then washed with phosphate buffer solution (PBS) and stained with fresh diaminobenzidine (DAB) solution. Finally, the sections were visualized under an Olympus optical microscope (Olympus Corporation, Tokyo, Japan). The appearance of brown colouring in the cytoplasm or cytomembrane of the tumour tissues was regarded as positive integrin αvβ3 expression.
Mouse anti integrin αvβ3 monoclonal antibody
Manufactured by Abcam
Sourced in United States
Mouse anti-integrin αvβ3 monoclonal antibody is a laboratory reagent used for the detection and quantification of the integrin αvβ3 protein in various biological samples. The antibody specifically binds to the αvβ3 integrin, which is a cell surface receptor involved in cell-cell and cell-extracellular matrix interactions.
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Lab products found in correlation
Optical microscope, by Olympus (1 mentions)
Alexa fluor 488 labeled goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Pcmv lifeact taggfp2, by Ibidi (1 mentions)
Recombinant sdf 1, by R&D Systems (1 mentions)
Slide chemotaxis3d, by Ibidi (1 mentions)
Nsc23766, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Y 27632, by Selleck Chemicals (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti integrin αvβ3 monoclonal antibody
1
Immunohistochemical Analysis of Integrin αvβ3 in HepG2 Tumors
2
Glioma Cell Migration Assay
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Glioma cell line U87 was purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Lipofectamine-3000™ and Alexa Fluor 488-labeled Goat Anti-Mouse IgG were purchased from Invitrogen (Carlsbad, CA, USA). The antibiotic G418 was purchased from Merck KGaA (Darmstadt, Germany). Plasmid pCMV-LifeAct-TagGFP2 and µ-Slide Chemotaxis3D were purchased from ibidi GmbH (Martinsried, Germany). The specific inhibitors of NSC23766 and Y27632 were purchased from Selleck Chemicals (Houston TX, USA). Recombinant SDF-1was purchased from R&D Systems (Minneapolis, MN, USA). 3D life dextran-CD hydrogel was purchased from CELLENDS (Germany). Mouse anti-integrin αVβ3 monoclonal antibody was purchased from Abcam (Cambridge, MA, USA).
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