The largest database of trusted experimental protocols

2 protocols using rhodamine b

1

Lipid-based Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[(polyethylene glycol)-1000] (DSPE-PEG1k), 1,2-dihexadecanoyl-rac-glycero-3-phosphocholine (DPPC) and cholesterol were purchased from Avanti Polar Lipids Inc. (Birmingham, AL, USA), IR1048 dye was bought from Sigma-Aldrich (Darmstadt, Germany), while a membrane protein extraction kit was ordered from Beyotime Biotechnology. In addition, 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI) was ordered from YEASEN Biotechnology (Shanghai) Co., Ltd. (Shanghai, China). The utilized consumables including radio-immunoprecipitation assay (RIPA) lysis buffer, phenylmethanesulfonyl fluoride (PMSF), phosphatase inhibitors, 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) and enhanced cell counting Kit-8 (CCK-8) were purchased from Beyotime Biotechnology. Further, hoechst 33342 was purchased from US EVERBRIGHT, Suzhou, China. Fluorescein isothiocyanate (FITC) and Rhodamine B were ordered from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Trypsin was bought from Gibco (Waltham, MA, USA). Calcein-AM and Propidium Iodide were ordered from Invitrogen (Waltham, MA, USA). In addition, all the chemicals used were not further purified. What is more, pure water used here was Milli-Q of 18.25 MΩ·cm (23 °C, EMD Millipore, Burlington, MA, USA).
+ Open protocol
+ Expand
2

Nanomaterial-based Cisplatin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tannic acid (TA), Pluronic® F68, cisplatin (CDDP) and Rhodamine B (RB) were purchased from Yuanye biotechnology company (Shanghai, China). Silver nitrate was obtained from aladdin reagent Co., Ltd. (Shanghai, China). Sodium Dodecyl Sulfonate (SDS) was bought from Sigma-Aldrich (USA). Phosphorylated H2AX (γH2AX) antibody was supplied by Cell Signaling Technology company. NOX4, and NAPDH antibody were purchased from Proteintech company. H2O2 fluorescent probe BES H2O2-Ac was got from Wako (Cat. #021-17801 (1 mg), Japan). MQAE probe and Fe2+ probe (Ferro Orange) were bought from Beyotime company (Shanghai, China) and Dong Ren company (Shanghai, China), respectively.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!