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Permount histological mounting medium

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Permount histological mounting medium is a transparent, resin-based product used to permanently mount and preserve microscope slides containing histological specimens. It provides a durable, long-lasting seal to protect the samples.

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Lab products found in correlation

Thionin acetate, by Merck Group (1 mentions) Hematoxylin, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Nbt bcip kit, by Roche (1 mentions) Abc reagent, by Vector Laboratories (1 mentions) Goat serum, by Vector Laboratories (1 mentions) Citrate buffer, by Vector Laboratories (1 mentions) Bloxall, by Vector Laboratories (1 mentions) Phosphate buffered saline (pbs), by Vector Laboratories (1 mentions) Ultraclear, by Avantor (1 mentions)

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Permount (1060 citations) Permount mounting medium (246 citations) Mounting medium (119 citations) Permount medium (30 citations)

5 protocols using permount histological mounting medium

1

Cryoprotection and Histological Sectioning

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All specimens measured for this study were immersion-fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer saline (PBS) for at least 1 week and then cryoprotected in a 30% sucrose solution in PBS until they sunk. Specimens were embedded in a 15% gelatin and 30% sucrose solution, placed into 4% PFA overnight, and then into 20% sucrose until the block sank. The embedded brains were sectioned on a sliding freezing microtome at a thickness of 40 μm in the sagittal or coronal plane and sections collected in PBS with 0.01% sodium azide. Every second section was mounted onto gelatinized slides, dehydrated through a graded ethanol series, cleared in Hemo-D, stained with thionin acetate (Sigma–Aldrich) and coverslipped with Permount histological mounting medium (Fisher Scientific).
Corfield J.R., Price K., Iwaniuk A.N., Gutierrez-Ibañez C., Birkhead T, & Wylie D.R. (2015). Diversity in olfactory bulb size in birds reflects allometry, ecology, and phylogeny. Frontiers in Neuroanatomy, 9, 102.
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2

Immunohistochemical analysis of IL-1β expression

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Kidneys were perfused in 4% paraformaldehyde, removed and fixed in 10% neutral buffered formalin. 5-μm tissue sections embedded in paraffin were cut and mounted onto microscope slides. Deparaffination was induced through heat, tissue cleaning agent, ethanol and water. To unmask antigen binding sites, slides were boiled in 0.01 M citrate buffer pH 6.0 for 20 minutes. Endogenous peroxidase activity was blocked by incubating slides in 3% H2O2 in 100% MeOH for 30 minutes. The sections were then incubated at room temperature for 30 minutes in 10% goat serum to block nonspecific binding and incubated overnight at 4°C in a humidified chamber with antibodies against IL-1β (Abcam, Cambridge, MA) diluted 1:50 in TBS-T containing 4% goat serum. Then, the slides were incubated for 30 minutes at room temperature in a humidified chamber with a biotinylated goat anti-rabbit IgG-B antibody diluted 1:200 in TBS-T. The slides were subsequently placed in streptavidin-horseradish peroxidase for 30 minutes at room temperature in a humidified chamber. Finally, the slides were incubated with 50 μL of diaminobenzadine (BioGenex, San Ramon, CA) as a substrate, counterstained with hematoxylin (Sigma-Aldrich, Saint Louis, MO), dehydrated, and fixed with Permount histological mounting medium (Fisher Scientific, Hampton, NH).
Conley S.M., Abais-Battad J.M., Yuan X., Zhang Q., Boini K.M, & Li P.L. (2017). CONTRIBUTION OF GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV2 TO NLRP3 INFLAMMASOME ACTIVATION IN MOUSE PODOCYTES DURING HYPERHOMOCYSTEINEMIA. Free radical biology & medicine, 106, 236-244.
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3

Indirect Immunolabeling of PCR Products

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We used an indirect immunolabeling method with a primary anti-digoxigenin antibody (Fab fragments; Roche) conjugated to alkaline phosphatase to detect the PCR product. Briefly, blocking was performed in 5% BSA (Sigma, USA) in PBS for 30 min. The slides were subsequently drained and an anti-DIG antibody (diluted 1:200 in 100 mM Tris-HCl, pH 7.4, and 150 mM NaCl) was applied (100 mL per sample) for 2 h at room temperature. The detection of alkaline phosphatase was performed for 10 min using an NBT/BCIP kit (Roche). After detection, the slides were rinsed in distilled water for 5 min and counterstained with Fast Green. The slides were air-dried and subsequently mounted in Permount histological mounting medium (Fisher Scientific, USA).
Medel-Flores O., Valenzuela-Rodríguez V.A., Ocadiz-Delgado R., Castro-Muñoz L.J., Hernández-Leyva S., Lara-Hernández G., Silva-Escobedo J.G., Vidal P.G, & Sánchez-Monroy V. (2018). Association between HPV infection and prostate cancer in a Mexican population. Genetics and Molecular Biology, 41(4), 781-789.
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4

Immunohistochemical Analysis of Uterine Tissues

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The collected uterine tissues were fixed in 4% paraformaldehyde and embedded in paraffin. Sections (5 μm) were immunostained (n = 5 per group)72 (link). Briefly, after deparaffinization, sections were rehydrated in an ethanol gradient, then boiled for 20 min in citrate-buffer (Vector Laboratories Inc., CA, USA) for antigen retrieval. Endogenous peroxidase activity was quenched with Bloxall (Vector Laboratories Inc., CA, USA), and tissues were blocked with 2.5% goat serum in PBS for 1 hr (Vector Laboratories Inc., CA, USA). After washing in PBS three times, tissue sections were incubated overnight at 4°C in 2.5% goat serum containing the primary antibodies listed in STAR Methods. Sections were incubated for 1 hr with biotinylated secondary antibody, washed, and incubated for 45 min with ABC reagent (Vector Laboratories Inc., CA, USA). Color was developed with 3, 3’-diaminobenzidine (DAB) peroxidase substrate (Vector Laboratories Inc.), and sections were counter-stained with hematoxylin. Finally, sections were dehydrated and mounted in Permount histological mounting medium (Fisher Scientific).
Wang E., Simard M., Bergeron Y., Beauchamp D, & Bergeron M.G. (2000). In Vivo Activity and Pharmacokinetics of Ziracin (SCH27899), a New Long-Acting Everninomicin Antibiotic, in a Murine Model of Penicillin-Susceptible or Penicillin-Resistant Pneumococcal Pneumonia. Antimicrobial Agents and Chemotherapy, 44(4), 1010-1018.
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5

Quantitative Histological Analysis of Cardiac Capillaries

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The left hearts were fixed in 10 % formalin and stored in 70% ethanol until sectioning. The hearts were cut in half transversally and dehydrated in increasing alcohol series (94% Ethanol, 3×100% ethanol) for 1–2 h depending on the tissue size and by incubation in UltraClear (J.T. Baker, Phillipsburg, NJ, USA, 2×1–2h) and then infiltrated with paraffin for 1–2 h and again in fresh paraffin overnight (Aldrich 76242). The samples were cut into 5-μm sections which were deparaffinized in UltraClear and rehydrated in decreasing alcohol series for capillary staining. The capillary staining was done using periodic acid-Schiff staining protocol according to Andersen (1975) using 35-min Schiff’s reagent staining (1.2 mM basic fuchsin; 0.1 M HCl; 2.1 mM sodium metabisulfite) [2 (link)]. After staining the samples, sections were dehydrated in increasing alcohol series and in UltraClear and then coverslipped using Permount histological mounting medium (Fisher, SO-P-15). All the imaging was done with Nikon Eclipse microscope using Nikon pE-300ultra camera and NIS-Elements AR-software. Histological images were analyzed using Image J 1.52a. The capillary density was counted from a minimum area of 20,000 μm2 of tissue. From these areas, the cell number was also determined which was used to count the average cell size and the capillary to cell ratio.
Uurasmaa T.M., Streng T., Alkio M., Heinonen I, & Anttila K. (2021). Short-term exercise affects cardiac function ex vivo partially via changes in calcium channel levels, without influencing hypoxia sensitivity. Journal of Physiology and Biochemistry, 77(4), 639-651.
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