PCR primers and TaqMan MGB probes for amplification of guinea pig SOD1-3, GPx1, GPx3, and GPx4 were designed using the Primer Express 2.0 software (Applied Biosystems, Foster City, CA, USA, 2001). The mRNA sequences were obtained from NCBI GenBank, and the corresponding accession numbers for each gene of interest is provided in Table 1 . The TaqMan gene expression assays for CAT and the housekeeping gene GAPDH for guinea pig were purchased from inventoried assays of Life Technologies (Carlsbad, CA, USA). The PCR primer and TaqMan probe sequences used to quantify the mRNA levels of the genes of interest are shown in Table 1 .
Taqman gene expression assays for cat
Manufactured by Thermo Fisher Scientific
Sourced in United States
TaqMan gene expression assays for CAT are a set of pre-designed and validated qPCR assays that target the catalase (CAT) gene. These assays are designed to quantify the expression levels of the CAT gene, which plays a crucial role in the detoxification of hydrogen peroxide within cells.
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2 protocols using taqman gene expression assays for cat
1
Quantifying Antioxidant Gene Expression in Guinea Pigs
2
Quantifying Guinea Pig Antioxidant Genes
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PCR primers and TaqMan MGB probes for amplification of guinea pig SOD1-3, GPx1, GPx3, and GPx4 were designed using the Primer Express 2.0 software (Applied Biosystems, Foster City, CA, USA, 2001). The mRNA sequences were obtained from NCBI GenBank, and the corresponding accession numbers for each gene of interest is provided in Table 1 . The TaqMan gene expression assays for CAT and the housekeeping gene GAPDH for guinea pig were purchased from inventoried assays of Life Technologies (Carlsbad, CA, USA). The PCR primer and TaqMan probe sequences used to quantify the mRNA levels of the genes of interest are shown in Table 1 .
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