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Alpha plan apochromat oil objective

Manufactured by Zeiss
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The 63× Alpha Plan-Apochromat oil objective is a high-performance microscope objective lens developed by Zeiss. It provides a 63x magnification and utilizes apochromatic design principles to deliver superior optical performance. The lens is optimized for use with immersion oil to achieve enhanced resolution and image quality.

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Lab products found in correlation

Lsm 880 confocal microscope, by Zeiss (4 mentions) Plan apochromat oil objective, by Zeiss (3 mentions) Lsm 710 confocal microscope, by Zeiss (3 mentions) Widefield system, by Nikon (2 mentions) Zen 2, by Zeiss (2 mentions) Csu x1 m1 spinning disk, by Sutter Instruments (2 mentions) Lsm 880 axioobserver, by Zeiss (1 mentions) Lsm 880 axioobserver confocal microscope, by Zeiss (1 mentions) Planapo n 60 tirf microscope objective, by Olympus (1 mentions) Ix83 microscope, by Olympus (1 mentions)

Spelling variants of this product

Plan-Apochromat (503 citations) Plan-Apochromat objective (157 citations) Plan-Apochromat oil objective (41 citations) Alpha Plan-Apochromat (13 citations) Alpha Plan-Apochromat TIRF 100x/1.6 NA oil objective (3 citations) Alpha Plan-Apochromat 100 × /1.46 Oil immersion Objective (3 citations) Alpha Plan-Apochromat objective 100×/1.46 Oil DIC M27 (3 citations) Alpha Plan-Apochromat 100 × /1.40 Oil immersion Objective (2 citations) Alpha-Plan-Apochromat ×63/1.46 oil objective lens (2 citations)

3 protocols using alpha plan apochromat oil objective

1

Super-resolution Imaging of Cellular Organelles

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Cells were maintained at 37°C and 5% CO2 (see Supplementary Data Table 2). Live cell Airyscan superresolution microscopy used a Zeiss LSM 880 confocal microscope with Airyscan detector and 63× Plan-Apochromat oil objective, NA 1.4. Sequential excitation of fluorophores was used, with 488nm laser line for Snap-Cell Oregon Green and MitoSOX, and 633nm laser for Snap-Cell 647-SiR, with appropriate dual bandpass filters. The Airyscan detector was adjusted regularly, and images were Airyscan processed in 2D using the Zeiss Zen 2.3 software package (Carl Zeiss Microscopy).
For spinning disk confocal imaging, cells were imaged on a 3i Marianas platform with a Zeiss Axio Observer Z1, Optovar 1×, 1.6× and 2.5×, with a Yokogawa CSU-X1 M1 spinning disk, Sutter LB-10W fast 10-position filter wheel with filters: 445/45, 525/30, 617/73, and Andor Neo sCMOS camera using a 63× Alpha Plan-Apochromat oil objective, NA 1.46 (Carl Zeiss Microscopy), using Slidebook 5.5 acquisition software (3i) and lasers: Violet (solid state 405nm/100mW), Blue (solid state 488nm/150mW), and Lime (solid state 561nm/50mW).
An inverted Nikon widefield system with Andor Zyla 4.2+ camera, external filter wheel and SpectraX LED was used for widefield imaging. Images were acquired using a 60× water objective, NA 1.20, and NIS-Elements 4.5 software.
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
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2

Detailed imaging protocol for microscopy

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Fixed samples were mounted on glass coverslips using Fluoromount-G and imaged using a confocal, widefield, or high-resolution Airyscan confocal microscopy as indicated in the figure legends. Widefield images were acquired using an Olympus IX-83 microscope using UPlanSAPO 40× silicon oil (NA 1.25; Fig. 2 A and Fig. S4 B) or PlanApo N 60× TIRF microscope objective (oil, NA 1.45; Fig. S2 C). Confocal images were acquired using a Zeiss LSM 710 confocal microscope with 63× Plan Apochromat oil objective (NA 1.4; Fig. 1 A and Fig. S1, D and E), Zeiss LSM 710 META with 63× Alpha Plan Apochromat oil objective (NA 1.46; Fig. 3, D and F), or Zeiss LSM880 Axio Observer with 63× Plan Apochromat oil objective (NA 1.4; Fig. 2 F and Fig. S2, A, B, D, and E). Airyscan confocal imaging was performed using Zeiss LSM 880 AxioObserver confocal microscope with Airyscan detector and 63× Plan Apochromat oil objective (NA 1.4). Images were acquired in superresolution scan mode (Fig. 1, B and E; and Fig. 5, A and B).
Collins K.B., Kang H., Matsche J., Klomp J.E., Rehman J., Malik A.B, & Karginov A.V. (2019). Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. The Journal of Cell Biology, 219(2), e201903023.
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3

Super-resolution Imaging of Cellular Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were maintained at 37°C and 5% CO2 (see Supplementary Data Table 2). Live cell Airyscan superresolution microscopy used a Zeiss LSM 880 confocal microscope with Airyscan detector and 63× Plan-Apochromat oil objective, NA 1.4. Sequential excitation of fluorophores was used, with 488nm laser line for Snap-Cell Oregon Green and MitoSOX, and 633nm laser for Snap-Cell 647-SiR, with appropriate dual bandpass filters. The Airyscan detector was adjusted regularly, and images were Airyscan processed in 2D using the Zeiss Zen 2.3 software package (Carl Zeiss Microscopy).
For spinning disk confocal imaging, cells were imaged on a 3i Marianas platform with a Zeiss Axio Observer Z1, Optovar 1×, 1.6× and 2.5×, with a Yokogawa CSU-X1 M1 spinning disk, Sutter LB-10W fast 10-position filter wheel with filters: 445/45, 525/30, 617/73, and Andor Neo sCMOS camera using a 63× Alpha Plan-Apochromat oil objective, NA 1.46 (Carl Zeiss Microscopy), using Slidebook 5.5 acquisition software (3i) and lasers: Violet (solid state 405nm/100mW), Blue (solid state 488nm/150mW), and Lime (solid state 561nm/50mW).
An inverted Nikon widefield system with Andor Zyla 4.2+ camera, external filter wheel and SpectraX LED was used for widefield imaging. Images were acquired using a 60× water objective, NA 1.20, and NIS-Elements 4.5 software.
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
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