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Cd62l fitc clone mel 14

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CD62L-FITC (clone MEL-14) is a fluorescently-labeled antibody that binds to the CD62L (L-selectin) cell surface receptor. CD62L is involved in lymphocyte homing and the inflammatory response. This product can be used for flow cytometric analysis of cells expressing CD62L.

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Lab products found in correlation

Cd3ε apc cy7 clone 145 2c11, by BioLegend (2 mentions) Cd44 peefluor610 clone im7, by BioLegend (2 mentions) Cd45 bv510 clone 30 f11, by BioLegend (2 mentions)

Close spelling variants of this product

CD62L (93 citations) CD62L (MEL-14, (36 citations) CD62L (clone MEL-14, (16 citations) MEL-14 (16 citations) CD62L-FITC (14 citations) CD62L-FITC (MEL-14; (4 citations)

2 protocols using cd62l fitc clone mel 14

1

Lung CD4+ Resident Memory T Cell Profiling

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Left (ipsilateral) and right (contralateral) lobes of uninfected Het Imm (i.t.) and saline mice were digested separately using collagenase as described above. Single-cell suspensions were prepared for flow cytometry and labeled with the following antibodies: CD45-BV510 (clone 30-F11), CD3ε-APC/Cy7 (clone 145-2C11), CD4-PE/Cy7 (clone GK1.5), CD11a-APC (clone M17/4), CD69-PE (clone H1.2F3), CD62L-FITC (clone MEL-14), and CD44-PEeFluor610 (clone IM7) (Biolegend). Lung resident memory CD4+ T cells were defined as living cells (L/D−) that were CD45+/CD3+/CD4+/CD11ahi/CD69+/CD62Llo/CD44hi. Data were expressed as % CD11ahiCD69+ cells of CD4+ T cells.
Smith N.M., Wasserman G.A., Coleman F.T., Hilliard K.L., Yamamoto K., Lipsitz E., Malley R., Dooms H., Jones M.R., Quinton L.J, & Mizgerd J.P. (2017). Regionally Compartmentalized Resident Memory T Cells Mediate Naturally Acquired Protection Against Pneumococcal Pneumonia. Mucosal immunology, 11(1), 220-235.
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2

Lung CD4+ Resident Memory T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Left (ipsilateral) and right (contralateral) lobes of uninfected Het Imm (i.t.) and saline mice were digested separately using collagenase as described above. Single-cell suspensions were prepared for flow cytometry and labeled with the following antibodies: CD45-BV510 (clone 30-F11), CD3ε-APC/Cy7 (clone 145-2C11), CD4-PE/Cy7 (clone GK1.5), CD11a-APC (clone M17/4), CD69-PE (clone H1.2F3), CD62L-FITC (clone MEL-14), and CD44-PEeFluor610 (clone IM7) (Biolegend). Lung resident memory CD4+ T cells were defined as living cells (L/D−) that were CD45+/CD3+/CD4+/CD11ahi/CD69+/CD62Llo/CD44hi. Data were expressed as % CD11ahiCD69+ cells of CD4+ T cells.
Smith N.M., Wasserman G.A., Coleman F.T., Hilliard K.L., Yamamoto K., Lipsitz E., Malley R., Dooms H., Jones M.R., Quinton L.J, & Mizgerd J.P. (2017). Regionally Compartmentalized Resident Memory T Cells Mediate Naturally Acquired Protection Against Pneumococcal Pneumonia. Mucosal immunology, 11(1), 220-235.
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