Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. In brief, hADSCs (1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING, Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture, which was then incubated on ice for 30 min. After washing the cells with Brilliant Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), FACSs measurement was carried out. The following primary antibodies was used: APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences); FITC anti-human CD90.2 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
Apc mouse anti human cd29
Manufactured by BD
Sourced in United States
The APC Mouse Anti-Human CD29 is a lab equipment product that detects the CD29 antigen, also known as Integrin beta-1, on the surface of human cells. It is used for the identification and analysis of CD29-expressing cells in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Novocyte flow cytometer, by Agilent Technologies (2 mentions)
Perfusion solution, by Corning (2 mentions)
Pe cy7 mouse igg1 κ isotype ctrl antibody, by BioLegend (2 mentions)
Pe cy7 anti human cd45 antibody, by BioLegend (2 mentions)
Brilliant stain buffer, by BD (2 mentions)
Facscanto 2, by BD (1 mentions)
Fitc mouse igg1 κ isotype ctrl antibody, by BioLegend (1 mentions)
3 protocols using apc mouse anti human cd29
1
Flow Cytometry Analysis of hADSCs
2
Characterization of Mesenchymal Stem Cells
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Prior to the respective experiment, control and transfected MSCs were cultivated in 25 cm2 flasks. Cells were detached by trypsinization, and resuspended in 2 mM EDTA/0.3% BSA/PBS solution. In the next step, cells were labeled using the following antibodies: APC Mouse IgG1, κ Isotype Control (555751, BD Pharmingen™), PE Mouse IgG1, κ Isotype Control (555749, BD Pharmingen™), APC Mouse Anti-Human CD29 (559883, BD Pharmingen™), PE Mouse Anti-Human CD49d (555503, BD Pharmingen™), washed and analyzed by BD FACSCanto II (BD Bioscences).
3
Characterization of hMSC-AT Markers
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Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences,
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
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