Apc123

The following primary antibodies were used:
Anti-CALD1 (mouse IgG1, MS-1251-P0, NeoMarkers, Fremont, CA, USA, 1:500), anti-COCH (rabbit IgG, HPA050122, Atlas Antibodies, Bromma, Sweden, 1:200), anti-CCN2 (goat IgG, sc14939, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:200), anti-ATP1B1 (mouse IgG2a, ab2873, Abcam, Cambridge, UK, 1:1000), anti-ATP1A1 (rabbit IgG, ab76020, Abcam, Cambridge, UK, 1:1000), anti-SLC12A2 (goat IgG, sc-21545, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:1000), anti-KCNJ16 (rabbit IgG, APC123, Alomone labs, Jerusalem, Israel, 1:1000), anti-CA2 (rabbit IgG, sc-25596, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:500), anti-GLUT1 (rabbit IgG, ab115730, Abcam, Cambridge, UK, 1:500), anti-COL2A1 (mouse IgG2b, sc-52658, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:100).
The following secondary antibodies were used: donkey anti-mouse IgG, Alexa Fluor Plus 488 (A32766, Invitrogen, Waltham, MA, USA, 1:500); donkey anti-rabbit IgG, Alexa Fluor Plus 555 (A32794, Invitrogen, Waltham, MA, USA, 1:500); and donkey anti-goat Alexa Fluor Plus 647 (A32849, Invitrogen, Waltham, MA, USA, 1:500).

The following antibodies were used for immunopanning, immunocytochemistry, immunohistochemistry and Western Blot experiments: goat anti-OLIG2 (AF2418, R and D Systems, 1:50), mouse anti-APC (clone CC1, OP80, 1:300, Millipore Sigma), mouse anti-NOGO-A (clone 11C7, gift from M.E. Schwab, 1:3,000), rat anti-MBP (ab7349, Abcam, 1:500), mouse anti-MOG (clone 8–18 C5, 1:1,000, Millipore Sigma), rat anti-GFAP (clone 2.2B10, 13–0300, Invitrogen, 1:1,000), rabbit anti-AQP4 (AB3594, 1:500, Millipore Sigma), rabbit anti-KIR4.1 (APC035, Alomone Labs, 1:3,000), rabbit anti-KIR4.1 (APC-165, Alomone Labs, 1:1,000), rabbit anti-KIR5.1 (APC123, Alomone Labs, 1:500), mouse anti-Neurofilament H (NF-H), nonphosphorylated (clone SMI32, 801701, Biolegend, 1:10,000), mouse anti-Neurofilament H (NF-H), phosphorylated (clone SMI312, 837904, Biolegend, 1:1,000), rabbit anti-IBA1 (019–19741, Wako, 1:500), goat anti-BRN3a (sc-31984, Santa Cruz, 1:200), rabbit anti-KCNQ2 (ab22897, Abcam, 1:200) rabbit anti-CASPR (ab34151, Abcam, 1:1,000), mouse anti-BRDU (347580, BD Biosciences, 1:200), rabbit anti-phospho-Histone H3 (pH3, 9701, Cell Signaling, 1:500), rat anti-CD140a (558774, BD Biosciences, 1:500), mouse anti-β-ACTIN (A5316, Sigma, 1:7,000).