2695 high performance liquid chromatography system

One precursor and the product ion(s) for each pesticide of interest were selected by first running the single MS full scan mode on a Waters 2695 high-performance liquid chromatography system coupled with a UV detector (Waters 996 photodiode array detector) and a Waters Acquity TQ triple quadrupole mass spectrometer (MS/MS) (Waters TQ Detector, Acquity ultra-performance LC). Subsequently, the product ion scan mode was performed. Within the Waters Empower 3 Chromatogaphy software, the AutoTune was performed on each individual analyte using the obtained precursor ion and the product ions. The MRM experimental optimal parameters were selected from the generated report and the precursor scan was used to determine retention time.

The spore suspension should be inoculated into Potato Dextrose Broth (PDB) culture medium and incubated at 25°C for 3 days. Follow the specific extraction method for ergosterol (Liu et al., 2013 (link)). The experiment should be conducted using a Waters 2,695 High Performance Liquid Chromatography system equipped with a Waters 2,996 UV detector. Ergosterol is separated on a Waters C18 column (5 μm, 4.6 mm × 250 mm) at 30°C, using 100% methanol (chromatographic grade) as the mobile phase. The detection wavelength is set at 282 nm (Dushkov et al., 2023 (link); Fernandes et al., 2023 (link); Manita et al., 2024 (link)). The standard for ergosterol is purchased from Shanghai YuanYue Biotechnology Co., Ltd. The experiment is repeated three times.