G13795 degasser

Manufactured by Agilent Technologies

Sourced in Ireland

The G13795 degasser is a laboratory equipment designed to remove dissolved gases from liquid samples. It functions by applying a vacuum to the liquid, which causes the gases to be extracted, resulting in a degassed sample.

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Lab products found in correlation

G1313a als, by Agilent Technologies (4 mentions) G1312a binpump, by Agilent Technologies (4 mentions) G1316a column oven, by Agilent Technologies (4 mentions) 1100 hplc system, by Agilent Technologies (3 mentions) Kinetex phenyl hexyl column, by Phenomenex (2 mentions) Allure pfp propyl column, by Restek (2 mentions) Single quadrupole msd, by Agilent Technologies (1 mentions)

4 protocols using g13795 degasser

Quantification of Analytes by LC-MS

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LC-MS analyses were performed on an Agilent 1100 HPLC system equipped with a G13795 degasser, G1312A BinPump, a G1313A ALS and G1316A column oven (COLCOM) (Agilent, Little Island, Cork, Ireland). Separation was obtained on a Kinetex phenyl-hexyl column (2.6 μm, 100 × 2.10 mm) Phenomenex (Macclesfield, Cheshire, United Kingdom). The analytes were eluted under isocratic conditions using amobile phase of 97%water and 3%acetonitrile (both containing 0.1% formic acid). The Agilent single quadrupole MSD settings were as follows: positive electrospray mode, capillary voltage 3500 V, drying gas (N2) 12 L/min at 350 °C, and nebulizer gas (N₂) pressure 50 psi. In-source collision-induced dissociation experiments were carried out with an increased fragmentor voltage of 110 V. Samples were dissolved in acetonitrile/water (1:1, containing 0.1% formic acid) at a concentration of 10 μg/mL. The injection volume was 0.5 μL, flow rate was 0.4 mL/min and the column temperature was set at 30 °C. Total run time was 25 min.

Brandt S.D., Kavanagh P.V., Dowling G., Talbot B., Westphal F., Meyer M.R., Maurer H.H, & Halberstadt A.L. (2016). Analytical characterization of seventeen ring-substituted N,N-diallyltryptamines. Drug testing and analysis, 9(1), 115-126.

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Quantitative LC-MS Analysis of Analytes

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LC-MS analyses were performed on an Agilent 1100 HPLC system equipped with a G13795 degasser, G1312A BinPump, a G1313A ALS and G1316A column oven (COLCOM) (Agilent, Little Island, Cork, Ireland). Separation was achieved using a Kinetex phenyl-hexyl column (2.6 μm, 100 x 2.10 mm) from Phenomenex (Macclesfield, Cheshire, United Kingdom). The analytes were eluted under isocratic conditions using a mobile phase of 95% water and 5% acetonitrile (both containing 0.1% formic acid). The Agilent LC-MSD settings were as follows: positive electrospray mode, capillary voltage 3500 V, drying gas (N₂) 12 L/min at 350 °C, nebuliser gas (N₂) pressure 50 psi, SIM m/z 192, fragmentor voltage 50 V and 150 V. Samples for LC-MS analysis were dissolved in acetonitrile/water (1:1, containing 0.1% formic acid) at a concentration of 10 μg/mL. The injection volume was 0.5 μL, flow rate was 0.2 mL/min and the column temperature was 30 °C. Total run time was 25 min.

McLaughlin G., Baumann M.H., Kavanagh P.V., Morris N., Power J.D., Dowling G., Twamley B., O’Brien J., Hessman G., Westphal F., Walther D, & Brandt S.D. (2018). Synthesis, analytical characterization and monoamine transporter activity of the new psychoactive substance 4-methylphenmetrazine (4-MPM), with differentiation from its ortho- and meta- positional isomers. Drug testing and analysis, 10(9), 1404-1416.

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LC-MS Analysis of Organic Compounds

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LC-MS analyses were performed on an Agilent 1100 HPLC system equipped with a G13795 degasser, G1312A BinPump, a G1313A ALS and G1316A column oven (COLCOM) (Agilent, Little Island, Cork). Separation was obtained on an Allure PFP Propyl column (5μm, 50 × 2.1 mm) Restek (Bellefonte, PA, USA). Mobile phase A consisted of 0.1% formic acid in water, whereas, mobile phase B consisted of 0.1% formic acid in acetonitrile. The Agilent LC-MSD settings were as follows: positive electrospray mode, capillary voltage 3500V, drying gas (N₂) 12L/min at 350°C, nebulizer gas (N₂) pressure 50 psi, scan mode m/z 70-500, fragmentor voltage 50 and 110V. Samples for LC-MS analysis were dissolved in acetonitrile/water (1:1, containing 0.1% formic acid) at a concentration of 10μg/mL. The injection volume was 10.0μL, flow rate was 0.8 mL/min and the column temperature was 30°C. Total run time was 25 min. The following gradient elution program was used: 0-2 min 2% B, followed by an increase to 60% B within 15 min, followed by another increase to 80% B within 18 min before returning to 2% B within 25 min.

McLaughlin G., Morris N., Kavanagh P.V., Power J.D., Dowling G., Twamley B., O'Brien J., Talbot B., Walther D., Partilla J.S., Baumann M.H, & Brandt S.D. (2016). Synthesis, characterization and monoamine transporter activity of the new psychoactive substance mexedrone and its N-methoxy positional isomer, N-methoxymephedrone. Drug testing and analysis, 9(3), 358-368.

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Quantification and Characterization of Clonazolam and Nitrazolam

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This was used for the quantification of clonazolam in the tablets and to obtain product ions spectra for clonazolam and nitrazolam utilizing in-source CID.
Analyses were performed on an Agilent 1100 HPLC system equipped with a G13795 degasser, G1312A BinPump, a G1313A ALS and G1316A column oven (COLCOM) (Agilent, Little Island, Cork). Separation was obtained on an Allure PFP Propyl column (5 μm, 50 x 2.1 mm) Restek (Bellefonte, PA, USA). Mobile phase A consisted of 0.1% formic acid in water, whereas mobile phase B consisted of 0.1% formic acid in acetonitrile. The flow rate was set at 0.80 mL/min, with an injection volume of 10 µL, and the column temperature was 30°C. The following gradient elution program was used: 0-2 min 2% B, followed by an increase to 60% B within 15 min, followed by another increase to 80% B within 18 min before returning to 2% B within 25 min (total run time was 25 min.). The Agilent LC-MSD mass spectrometer settings were as follows: positive electrospray mode, capillary voltage 3500 V, drying gas (N 2 ) 12 L/min at 350 °C, nebulizer gas (N 2 ) pressure 50 psi, and scan mode m/z 70-500.
To obtain product ions spectra, samples were dissolved in acetonitrile/water (1:1, containing 0.1% formic acid) and the fragmentor voltage was set at 150 V. For the quantification of clonazolam in tablets, the fragmentor voltage was set at 50 V.

Dowling G., Kavanagh P.V., Eckhardt H.G., Twamley B., Hessman G., McLaughlin G., O'Brien J, & Brandt S.D. (2018). An approach to shortening the timeframe between the emergence of new compounds on the drugs market and the availability of reference standards: The microscale syntheses of nitrazolam and clonazolam for use as reference materials, utilizing polymer-supported reagents. Drug testing and analysis.

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