Aron alpha a tissue adhesive

The simple dialysis-culture system consists of upper culture compartment and lower dialysate compartment. A mesh bottom-cell strainer (PluriSelect, Leipzig, Germany) was modified by cutting and replacing the bottom mesh layer with a 12-kDa MWCO Spectra/Por 4 dialysis membrane (Spectrum Chemical, New Brunswick, NJ, USA). The dialysis membrane was affixed to the bottom of the strainer using an alkyl-α-cyanoacrylate-based surgical-grade tissue adhesive (Aron Alpha A; Daiichi Sankyo, Japan). The upper dialysis culture compartment inserts were then placed in 6-deep well plates (Corning, NY, USA). To represent the control condition, the cell strainer was directly affixed to the bottom surface of six-well-plates (Iwaki, Tokyo, Japan) using Aron Alpha A tissue adhesive (Daiichi Sankyo). All the devices were sterilized using an ethylene oxide gas sterilizer before use.

The dialysis-culture system included upper and lower dialysate compartments (Fig. 1a). A 40-µm mesh bottom-cell strainer (PluriSelect, Leipzig, Germany) was used as the upper culture compartment, with this insert modified by cutting and removing the bottom mesh layer. To selectively permeate nutrition or waste products, we affixed a 12-kDa MWCO Spectra/Por 4 dialysis membrane (Spectrum Chemical, New Brunswick, NJ, USA) to the bottom side of the strainer using alkyl-α-cyanoacrylate-based surgical-grade tissue adhesive (Aron Alpha A; Daiichi Sankyo, Japan). The upper compartment was then placed in six-deep well plates (Corning, NY, USA) as dialysate-compartment inserts. As a control condition, the cell strainer was directly affixed to the bottom surface of six-well untreated plates (Iwaki, Tokyo, Japan) using Aron Alpha A tissue adhesive (Daiichi Sankyo). All devices were sterilized using an ethylene oxide gas sterilizer before use. The detailed device construction was explained in Supplementary method 1.