Pbgpa cmv gfp osbp ph domain

Manufactured by Addgene

The PBGPa-CMV-GFP-OSBP PH domain is a lab equipment product that provides a specific domain from the oxysterol-binding protein (OSBP). This domain is commonly used in research applications to study lipid-binding interactions and cellular signaling pathways.

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Lab products found in correlation

Mcherry rab5, by Addgene (1 mentions) Mcherry rab11a, by Addgene (1 mentions) Gfp p4m sidm, by Addgene (1 mentions) Gfp p4m sidmx2, by Addgene (1 mentions) Ph btk gfp, by Addgene (1 mentions) Mkate2 p2a apex2 tapp1 ph, by Addgene (1 mentions) Akt ph gfp, by Addgene (1 mentions) Ph plcd1 gfp, by Addgene (1 mentions) Ph plcd1 r40l gfp, by Addgene (1 mentions)

2 protocols using pbgpa cmv gfp osbp ph domain

Fluorescent Protein Constructs for Cellular Imaging

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Expression constructs encoding for human flotillin-1 or flotillin-2 were a gift from V. Niggli (University of Bern). TCRζ-PSCFP2 was provided by Prof. K. Gaus (University of New South Wales). PA-mCherry expression backbone was obtained from Clontech. TCRζ PA-mCherry was made as described in [15 (link)]. mCherry-Rab5 was a gift from Gia Voeltz (Addgene plasmid # 49,201). mCherry-Rab11a was a gift from Michael Davidson (Addgene plasmid # 55,124). pBGPa-CMV-GFP-OSBP PH domain was a gift from Tim Levine (Addgene plasmid # 58,724). LACT-C2 and 2xFYVE expression constructs were a gift from Prof. Rob Parton (University of Queensland).

Rossatti P., Redpath G.M., Ziegler L., Samson G.P., Clamagirand C.D., Legler D.F, & Rossy J. (2022). Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation. BMC Biology, 20, 189.

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Plasmid Transfection and Infection in RAW264.7 Cells

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The following probes GFP-P4M-SidM (Addgene plasmid # 51469); GFP-P4M-SidMx2(Addgene plasmid #51472); PH-Akt-GFP (Addgene plasmid # 51465); PH-Akt(R25C)-GFP (Addgene plasmid # 51466) PH-PLCD1-GFP (Addgene plasmid # 51407); PH-PLCD1(R40L)-GFP (Addgene plasmid # 51408); PH-Btk-GFP (Addgene plasmid # 51463); PH-Btk(R28C)-GFP (Addgene plasmid # 51464) were gifts from Tamas Balla; GFP-EEA1 wt (Addgene plasmid # 42307) was a gift from Silvia Corvera; pBGPa-CMV-GFP-OSBP PH domain (Addgene plasmid # 58840) was a gift from Tim Levine & Sean Munro. mKate2-P2A-APEX2-TAPP1-PH (Addgene Plasmids #67662) was a gift was a gift from Rob Parton. Plasmids were isolated and test digested for confirmation. They were then introduced into RAW264.7 by nucleofection using reagents from Mirus Bio (Madison, Wisconsin). After seeding transfected cells and overnight culture, they were infected. Infection of transfectants were evaluated after 2 or 24hr.

Zhang N., Prasad S., Despointes C.E., Young J, & Kima P.E. (2018). Leishmania parasitophorous vacuole membranes display phosphoinositides that create conditions for continuous Akt activation and a target for miltefosine in Leishmania infections. Cellular microbiology, 20(11), e12889.

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