Cmp n acetylneuraminic acid

Manufactured by Merck Group

CMP-N-acetylneuraminic acid is a laboratory reagent used in the analysis and synthesis of sialic acids. It serves as a precursor for the enzymatic transfer of N-acetylneuraminic acid to glycoconjugates. The product is intended for research use only and its detailed applications should be evaluated by the user.

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Lab products found in correlation

2 protocols using cmp n acetylneuraminic acid

Enzymatic Synthesis of Glycosidic Substrates

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Substrates were obtained from the following sources: UDP-α-d-xylose, UDP-β-l-arabinopyranose and UDP-α-d-galacturonic acid (Carbosource Services, Complex Carbohydrate Research Center, Athens, GA); UDP-α-d-glucuronic acid, UDP-α-d-glucose, UDP-α-d-galactose, UDP-N-acetyl-α-d-glucosamine, UDP-N-acetyl-α-d-galactosamine, GDP-α-d-mannose, GDP-β-l-fucose, GDP-α-d-glucose, adenosine 3′-phosphate 5′ phosphosulfate, CMP-N-acetylneuraminic acid and ADP-α-d-glucose (Sigma-Aldrich, St Louis, MO); and UDP-β-l-arabinofuranose (Peptides International, Louisville, KY). UDP-β-l-rhamnose was enzymatically synthesised as described earlier (18 (link)).

Rautengarten C., Quarrell O.W., Stals K., Caswell R.C., De Franco E., Baple E., Burgess N., Jokhi R., Heazlewood J.L., Offiah A.C., Ebert B, & Ellard S. (2019). A hypomorphic allele of SLC35D1 results in Schneckenbecken-like dysplasia. Human Molecular Genetics, 28(21), 3543-3551.

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Synthesis of Nucleotide Sugars

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D-ribose, D-ribulose, and D-ribitol were purchased from Carbosynth. D-galactose was from Merck Millipore. Cytidine triphosphate, D-xylulose, D-xylose, L-arabinose, doxycycline, and 1 mol/L triethylammonium acetate were from Sigma. CMP-N-acetylneuraminic acid, UDPgalactose, UDP-glucose, UDP-N-acetylgalactosamine, UDP-N-acetylglucosamine, GDP-mannose, GDP-fucose, ADP-ribose, UDP-xylose, and UDP-glucuronic acid were from Sigma or Carbosynth.
Rbo5P was produced as previously described (5 ) . CDP-ribitol was synthesized in vitro from Rbo5P and Cytidine triphosphate with recombinantly produced human CRPPA enzyme from Escherichia coli (kind gift of Dr. W. Yue, Oxford) (5 ) . The internal standard, a 13 Cyeast extract containing [U- 13 C]-UDP-glucose, was a kind gift of Dr. G. Hermann, Vienna.

van Tol W., van Scherpenzeel M., Alsady M., Riemersma M., Hermans E., Kragt E., Tasca G., Kamsteeg E.J., Pennings M., van Beusekom E., Vermeulen J.R., van Bokhoven H., Voermans N.C., Willemsen M.A., Ashikov A, & Lefeber D.J. (2019). Cytidine Diphosphate-Ribitol Analysis for Diagnostics and Treatment Monitoring of Cytidine Diphosphate-l-Ribitol Pyrophosphorylase A Muscular Dystrophy. Clinical chemistry, 65(10).

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