Anti lxrα

Proteins were extracted from mouse tissues or cultured cells using RIPA buffer (Biocolor Ltd., Belfast, Northern Ireland, UK), quantified using the BCA protein assay kit (KeyGen Biotechnologies, Nanjing, China), and then subjected to western blot analyses (10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis; 30 μg protein per lane) using rabbit polyclonal anti-APOM antibodies (BD Bio-sciences, San Jose, CA, USA), rabbit polyclonal anti-Foxa2 antibodies (Epitomics., CA, USA), and rabbit polyclonal anti-LXRα (Proteintech group, Inc., Chicago, IL, USA) and β-actin-specific antibodies (Abcam Inc.,Cambridge, MA, USA). The proteins were visualized using a chemiluminescence method (ECL Plus Western Blot Detection System; Amerisham Biosciences, Foster City, CA, USA).

Cholesterol, T0901317, GW3965, LXR-623, and SR9243 were ordered from MedChemExpress (Monmouth Junction, NJ, USA). Filipin complex and 22-R-hydroxycholesterol were ordered from Sigma-Aldrich (St. Louis, MO, USA). Anti-LXRα, anti-LXRβ, anti-AP2B, and anti-β-actin were ordered from Proteintech (Rosemont, IL, USA); anti-ABCA1 was ordered from Novus Biologicals (Littleton, CO, USA); Alexa-Fluor-488-conjugated goat anti-mouse IgG, Alexa-Fluor-568-conjugated goat anti-mouse IgG, and Alexa-Fluor-568-conjugated goat anti-rabbit IgG were ordered from Thermo Fisher Scientific (Waltham, MA, USA). Antiserum against PRV glycoprotein gB and gE was generated by immunization of mice with purified recombinant gB and gE.