Sodium dodecyl sulfate (sds)

N-Vinylcaprolactam (VCL) was obtained from Aldrich; N,N′-methylenebisacrylamide (BIS) (99%) from Sigma-Aldrich; N,N,N′,N′ tetramethylethylenediamine (TEMED) (98%) from Fluka; potassium persulfate (KPS) from Merck; sodium dodecyl sulfate (SDS) (96%) from BioPack; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from Sigma-Aldrich; dimethyl sulfoxide (DMSO) from Sigma-Aldrich; nuclease-free water from Promega, Madrid, Spain; phosphate-buffered saline (PBS) from Lonza, Walkersville, MD, USA; Dulbecco’s Modified Eagle’s Medium (DMEM) from Biochrom, AG, Germany; supplemented with 5% heat-inactivated-fetal bovine serum from Life Technologies, Madrid, Spain and a cocktail of antibiotics. Dialysis membranes of cellulose ester with a molecular weight cut-off of 50 kDa were purchased from SpectrumLabs. All chemicals were used as purchased. MilliQ grade water was employed as the polymerization medium.

The antibiotic G418 and the culture medium Dulbecco′s Modified Eagle′s Medium (DMEM) were from Sigma-Aldrich (St. Louis, MO, USA). The CellTiter 96^®Aqueous One Solution Cell Proliferation Assay kit was from Promega (Tecnolab, Buenos Aires, Argentina). Ferric chloride hexahydrate, sodium dodecyl sulfate (SDS), and hematoxylin and eosin (H&E) dyes were provided by Biopack (Buenos Aires, Argentina). Ferrous sulfate heptahydrate and potassium ferrocyanide were from Mallinckrodt Chemical Works (Saint Louis, MO, USA). Sodium hydroxide and acetic acid were purchased from Cicarelli (San Lorenzo, Santa Fe, Argentina). Neutral red (3-amino-7-dimethylamino-2-methyl-phenazine hydrochloride), 2′,7′-dichlorofluorescin diacetate (DCFDA), osmium tetroxide, uranyl acetate, lead citrate, and Spurr’s resin were acquired by Sigma (Sigma-Aldrich, Saint Louis, MO, USA). All other reagents were of analytical grade.