AP67 and AP72 were kind gifts from Dr. Whiteman's laboratory. Propargylglycine (PAG), aminooxyacetic acid (AOAA), NaHS, S-adenosyl-L-methionine (SAM), glibenclamide and L-nitroarginine methyl ester (L-NAME) were all purchased from Sigma Aldrich, St. Louis, MO. Flurbiprofen was procured from Cayman Chemicals, Ann Arbor, MI. All test agents were freshly prepared immediately before use on the day of the study. Stock solution of NaHS, AP67 and AP72 were prepared in distilled water whilst all other compounds were prepared in 70% ethanol. Vehicle controls were prepared using 0.1% saline.
Flurbiprofen
Manufactured by Cayman Chemical
Sourced in United States
Flurbiprofen is a synthetic organic compound used as a laboratory standard. It is a member of the phenylpropionic acid class of nonsteroidal anti-inflammatory drugs (NSAIDs). Flurbiprofen is commonly used as a reference material for analytical and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Aminooxyacetic acid, by Merck Group (1 mentions)
Glibenclamide, by Merck Group (1 mentions)
Propargylglycine, by Merck Group (1 mentions)
L nitroarginine methyl ester l name, by Merck Group (1 mentions)
S adenosyl l methionine, by Merck Group (1 mentions)
Sodium chloride (nacl), by Avantor (1 mentions)
Potassium phosphate monobasic, by Avantor (1 mentions)
Ketorolac, by Cayman Chemical (1 mentions)
Sodium phosphate dibasic, by Avantor (1 mentions)
Tween 20, by Thermo Fisher Scientific (1 mentions)
Branson sonifier 250, by Emerson (1 mentions)
Poloxamer 407, by BASF (1 mentions)
4 protocols using flurbiprofen
1
Modulation of Hydrogen Sulfide Signaling
2
Pharmacological Protein Purification Protocol
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Sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), and acetonitrile were all purchased from VWR International (Ville Mont-Royal, QC, Canada). Anti-polyethylene glycol antibody [PEG-B-47] was obtained from Abcam (Toronto, ON, Canada). SureBeads magnetic beads with protein A were bought from Biorad Laboratories (Mississauga, ON, Canada). Tween-20 was purchased from Fisher Scientific (Ottawa, ON, Canada). Flurbiprofen and ketorolac were obtained from Cayman Chemical Company (Ann Harbour, MI, USA).
3
Leptin-Flurbiprofen Interaction Protocol
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Flurbiprofen was obtained from Cayman Chemical (MI). Human leptin was obtained from Sigma (L4146; St. Louis, MO, USA) or ENZO Life Sciences (SE-161; Plymouth Meeting, PA, USA).
4
Lipid Carrier Nanoparticles for Drug Delivery
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Materials Flurbiprofen was purchased from Cayman Chemical (Ann Arbor, MI, U.S.A.). 1-Glyceryl monooleyl ether (GME) and hydrogenated lecithin (HL) were kindly donated by Nikko Chemicals Co., Ltd. (Tokyo, Japan). Poloxamer 407 and 1,3-butylene glycol (1,3-BG) were obtained from BASF Japan Ltd. (Tokyo, Japan) and Wako Pure Chemical Industries, Ltd. (Tokyo, Japan), respectively. All other chemicals used were of reagent grade.
Preparation of LCNs GME and Flurbiprofen were dissolved in ethanol. HL was dissolved in chloroform. These solutions were then mixed in the sample tube at the appropriate molar ratio. The organic solvents were removed by rotary evaporation (CVE-200D, Tokyo Rikakikai Co., Ltd., Tokyo, Japan) under vacuum at 30°C. The dry lipid film was hydrated with 1 mL of 50 mM citrate buffer (pH 3.0) containing the appropriate amounts of 1,3-BG and Poloxamer 407 (1%). For the LCNs prepared for NMR measurements, the film was hydrated with 50 mM citrate buffer (pH 3.0) using D 2 O, which contained 1,3-BG and Poloxamer 407. The suspensions were subsequently sonicated for 1 min using a Branson Sonifier 250 (Branson Ultrasonics, Emerson Japan Ltd., Kanagawa, Japan). During preparation, sample tubes were cooled with ice.
Preparation of LCNs GME and Flurbiprofen were dissolved in ethanol. HL was dissolved in chloroform. These solutions were then mixed in the sample tube at the appropriate molar ratio. The organic solvents were removed by rotary evaporation (CVE-200D, Tokyo Rikakikai Co., Ltd., Tokyo, Japan) under vacuum at 30°C. The dry lipid film was hydrated with 1 mL of 50 mM citrate buffer (pH 3.0) containing the appropriate amounts of 1,3-BG and Poloxamer 407 (1%). For the LCNs prepared for NMR measurements, the film was hydrated with 50 mM citrate buffer (pH 3.0) using D 2 O, which contained 1,3-BG and Poloxamer 407. The suspensions were subsequently sonicated for 1 min using a Branson Sonifier 250 (Branson Ultrasonics, Emerson Japan Ltd., Kanagawa, Japan). During preparation, sample tubes were cooled with ice.
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