After 24 h incubation in 30 °C, the cells were centrifuged and removed from the culture medium with saline. The inoculum suspension in saline with McFarland density of 0.5 was carefully spread on Petri plates with 4-mm thick MRS agar. When the suspension was absorbed by the agar, rings with antibiotics were distributed in triplicate. Erythromycin E15, kanamycin K30, bacitracin B10, streptomycin S10, amoxycillin AML25, tetracycline TE30, trimethoprim WE, penicillin P10, pirlimycin PIR2, chloramphenicol C30, and nalidixic acid NA30 were purchased from Oxoid (Hampshire, England).
Nalidixic acid na30
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Nalidixic acid NA30 is a laboratory reagent used for antimicrobial susceptibility testing. It is a quinolone antibiotic that inhibits bacterial DNA gyrase, a critical enzyme for bacterial replication. Nalidixic acid NA30 is typically used in disc diffusion or gradient diffusion methods to determine the antibiotic susceptibility of bacterial isolates.
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Lab products found in correlation
Erythromycin e15, by Thermo Fisher Scientific (2 mentions)
Streptomycin s10, by Thermo Fisher Scientific (2 mentions)
Penicillin p10, by Thermo Fisher Scientific (2 mentions)
Chloramphenicol c30, by Thermo Fisher Scientific (2 mentions)
Levofloxacin lev 5, by Thermo Fisher Scientific (1 mentions)
Nitrofurantoin f300, by Thermo Fisher Scientific (1 mentions)
Ciprofloxacin cip 5, by Thermo Fisher Scientific (1 mentions)
2 protocols using nalidixic acid na30
1
Antibiotic Susceptibility Assay
2
Antimicrobial Susceptibility of Bacterial Pathogens
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The antimicrobial sensitivity test of all isolated Salmonella, E. coli, and Staphylococcus strains was conducted according to Koneman et al. [14 ] by the disc diffusion method, and different antibiotic discs were used such as ampicillin Amp10, chloramphenicol C30, ciprofloxacin CIP5, colistin sulfate CT25, danofloxacin DX, doxycycline Do30, enrofloxacin ENR5, erythromycin E15, fosfomycin FOS-200, levofloxacin LEV-5, nalidixic acid NA30, norfloxacin NOR10, nitrofurantoin F300, penicillin P10, streptomycin S10, tetracycline T30, and trimethoprim-sulfamethoxazole 25 SXT; Oxoid. The results were interpreted according to the Clinical and Laboratory Standards Institute [15 ].
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