Mcf 10a cell medium

The human MCF7, SK-BR3, BT549 and MDA-MB-231 BC cell lines, and the normal MCF10A mammary epithelial cell line were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. SK-BR3, MCF-7, BT549 and MDA-MB-231 cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) penicillin/streptomycin solution (Beyotime Institute of Biotechnology). MCF10A cells were grown in MCF-10A cell medium (Procell Life Science & Technology Co., Ltd.). Standard cell culture techniques were used to grow and passage all cell lines in an incubator at 37°C in a 5% CO₂ atmosphere.

The MCF7, SK-BR3 and BT549 BC cell lines, which are derived from humans, were obtained from The Cell Bank of the Type Culture Collection of The Chinese Academy of Sciences. The MCF10A mammary epithelial cell line, which is considered normal, was also acquired from the same source. The SK-BR3, MCF-7 and BT549 cells were grown in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin solution. The MCF10A cells, on the other hand, were cultured using MCF-10A cell medium (Procell Life Science & Technology, Co., Ltd.). All cell lines were maintained and passaged in a standard cell culture incubator at a temperature of 37°C and a 5% CO₂ environment in an atmosphere with saturated humidity.