Htb 130

The cell lines were MDA-MB-436 (ATCC® HTB-130™) and MDA -MB 231 (ATCC® HTB 26™), both cell lines of human TNBT of the transcriptional claudin-low subtype [20 ] (ATCC. USA). They were maintained and expanded in DMEM-F-12 culture medium (Sigma) supplemented with 4.5% glucose, 4 mM l-glutamine, 1% antibiotics (Merck-Millipore), 1% essential amino acids (MEM, Sigma), and 10% foetal bovine serum. The difference between the cell cultures was that MDA-MB-436 cells were also supplemented with 0.01 mg/ml insulin (Sigma) and 16 mcg/ml glutathione (Sigma).

Human TNBC cell lines HS578T (American Type Culture Collection (ATCC) HTB-126™), MDA-MB-231 (ATCC HTB-26™), and MDA-MB-436 (ATCC HTB-130™) were used as the TNBC cell model in this study. HS578T cells were cultured in Dulbecco’s Modified Eagle’s Medium, while both MDA-MB-231 and MDA-MB-436 cells were cultured in Leibovitz’s L-15 medium. All culture media were supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin. In addition, the culture media for both HS578T and MDA-MB-436 cells were replenished with 10 µg/mL insulin, and 16 µg/mL glutathione was particularly added to MDA-MB-436 cell culture. Cells were grown at 37 °C in a humidified environment with 5% CO₂ for culturing HS578T cells or without 5% CO₂ for the growth of MDA-MB-231 and MDA-MB-436 cells.