Acquity uplc h class pda

All chemicals or reagents used for syntheses were of analytical reagent, and used directly without purification. Melting points (m.p.) were determined on a RY-2 apparatus and are uncorrected. ¹H NMR spectra were recorded on a Brucker spectrometer at 600 MHz with the CDCl₃ as the solvent and TMS as the internal standard. ¹³C NMR spectra were recorded on a Brucker spectrometer at 150 MHz with CDCl₃ as the solvent. Mass spectra were performed on a Waters ACQUITY UPLC^® H-CLASS PDA (Waters^®) instrument. Column chromatography was carried out using silica gel 100–200 mesh. Analytical thin-layer chromatography (TLC) was carried out on precoated plates, and spots were visualized with ultraviolet light.

All starting materials and reagents commercially available were used without further purification, unless otherwise specified. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Avance III 600 MHz FT-NMR spectrometer using DMSO-d₆ as the solvent and tetramethylsilane (TMS) as the internal standard. Chemical shifts are reported in δ (parts per million) values, and coupling constants ⁿJ are reported in Hz. Mass spectra were performed on a Waters ACQUITY UPLC^® H-CLASS PDA (Waters^®) instrument. Analytical thin-layer chromatography was carried out on precoated silica gel plates GF254 (Qindao Haiyang Chemical, China), and spots were visualized with ultraviolet light.

¹H-NMR spectra were recorded on a Bruker spectrometer at 600 (Bruker, Bremen, Germany) with DMSO-d₆ as the solvent and TMS as the internal standard; ¹³C-NMR spectra were recorded on a Bruker spectrometer at 150 MHz with DMSO-d₆ as the solvent. Chemical shifts are reported in δ (parts per million) values, and coupling constants ⁿJ are reported in Hz. Mass spectra were performed on a Waters ACQUITY UPLC® H-CLASS PDA (Waters®, Milford, MA, USA) instrument. Analytical thin-layer chromatography (TLC) was carried out on precoated plates, and spots were visualized with ultraviolet light. All chemicals or reagents used for syntheses were commercially available, were of AR grade, and were used as received.