Evans blue

Palbociclib was purchased from Med Chem Express (Monmouth Junction, NJ). Monoclonal DNP-specific IgE, DNP-HSA, and 4-nitrophenyl N-acetyl-β-D-glucosaminide were obtained from Sigma-Aldrich (St. Louis, MO). Evans blue, formamide, toluidine blue and mast cell stabilizer ketotifen were obtained from Dalian Meilun Biotechnology Co. Ltd. (Dalian, China). Antibodies targeting the tyrosine-protein kinase Lyn, Tyr397 phosphosphorylated (p)-Lyn, mitogen-activated protein kinase (MAPK) p38, c-Jun N-terminal kinase (JNK) (Abcam, Cambridge, MA), extracellular signal-regulated kinase (ERK)1/2, p-p38 (Thr180/Tyr182), p-JNK (Thr183/Tyr185), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Santa Cruz Biotechnology, Santa Cruz, CA), and p-ERK1/2 (Thr202/Tyr204) (p-ERK1/2) (Cell Signaling Technology, Beverly, MA) were used. Fluorescein isothiocyanate (FITC)-phalloidin was purchased from Yeasen Biotech Co. Ltd. (Shanghai, China). Lyn inhibitor Bafetinib, ERK inhibitor U0126, JNK inhibitor SP600125 and p38 inhibitor SB203580 were purchased from MedChem Express (Monmouth Junction, NJ, USA). Silencing RNAs (SiRNAs) for knocking down the expression of Lyn were designed and obtained from Gene Pharma (Shanghai, China).

FNT (formononetin) was purchased from TargetMol (Boston, MA, USA). 4-nitrophenyl N-acetyl-β-D-glucosaminide was purchased from Sigma (St. Louis, MO, USA). Dexamethasone (Dexa), ketotifen fumarate (Keto), Evans blue, and toluidine blue were purchased from Meilun Biotechnology (Dalian, China). Primary rabbit antibodies against IκBα (ab76429, monoclonal), p-IκBα (ab133462, monoclonal), p65 (ab32536, polyclonal), GAPDH (ab181602, monoclonal), and loricrine (ab176322, monoclonal) were purchased from Abcam (Cambridge, MA, USA). Antibodies targeting filaggrin (DF13853, monoclonal) were purchased from Affinity Biosciences (Cincinnati, OH, USA). Anti-p-p65 (sc-135769, monoclonal), anti-rabbit IgG-HRP (sc-2357, monoclonal), and 4% paraformaldehyde solution in PBS were from Santa Cruz Biotechnology (Dallas, TX, USA). 2,4-dinitrochlorobenzene (DNCB) was purchased from Tokyo Chemical Industry (Tokyo, Japan). C48/80 was purchased from Cayman Chemical (Ann Arbor, MI, USA).

The reagents 4-MU (CID: 5280567), ketotifen fumarate (CID: 5282408), Evans blue (CID: 9409), toluidine blue (CID: 7083), and NF-κB inhibitor BAY 11-7082 (CID: 5353431) were purchased from Meilun Biotechnology Co., Ltd. (Dalian, China). ERK inhibitor U0126 (CID: 3006531), JNK inhibitor SP600125 (CID: 8515), and p38 inhibitor SB203580 (CID: 176155) were purchased from MedChem Express (Monmouth Junction, NJ, USA). DNP-HSA, monoclonal DNP-specific IgE and OVA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Aluminum adjuvant was purchased from Thermo Scientific (Waltham, MA, USA). Rabbit monoclonal IgG primary antibodies against JNK (c-Jun N-terminal kinase), phosphorylated (p)-JNK (Thr183/Tyr185), p-SYK (spleen tyrosine kinase, Tyr525/ Tyr526), ERK (extracellular signal-regulated kinase), p-ERK (Thr202/Tyr204), p38, p-p38 (Thr180/Tyr182), and anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (Cell Signaling Technology, Danvers, MA). We bought rabbit monoclonal primary antibodies targeting SYK, p65, and IκBα from Abcam (Cambridge, MA) and bought IgGκ BP-horse radish peroxidase (HRP), anti-rabbit IgG-HRP, and monoclonal IgG anti-p-p65 (Ser311) primary antibodies from Santa Cruz Biotechnology (Dallas, TX, USA).