A FluoroNunc Maxisorp 96-well plate was coated with 100 µg/mL salmon sperm dsDNA (AM9680, ThermoFisher Scientific) in PBS and incubated overnight at 4 °C. Wells were blocked with 200 µL TBS containing 1% bovine serum albumin (BSA) (A4503, Sigma-Aldrich) for 1 hour at RT and washed thrice with TBS/Tw (TBS containing 0.05% v/v Tween-20 (8.17072.1000, Merck)). Samples, standards, and quality controls were diluted in TBS/Tw containing 5 mM EDTA and 0.1% w/v BSA, and subsequently added in duplicates. The plate incubated at 37 °C for 1 hour. Wells were washed thrice in TBS/Tw and incubated with biotinylated goat-anti-mouse Ig (1010-08, Southern Biotech), 1 µg/ml TBS/Tw, at 37 °C for 1 hour. Wells were washed 3 times in TBS/Tw, and Eu3+-labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 µM EDTA was subsequently added to the wells and incubated at RT for 1 h. Finally, the wells were washed thrice with TBS/Tw, and 200 µL enhancement buffer (AMPQ99800, Amplicon) was added. The plate was shaken for 5 min and counts were read in a time-resolved fluorometry plate reader (Victor X5, PerkinElmer).
Biotinylated goat anti mouse ig 1010 08
Manufactured by Southern Biotech
Biotinylated goat-anti-mouse Ig (1010-08) is a laboratory reagent used for the detection and analysis of mouse immunoglobulins (Ig) in various immunological assays. This product consists of goat-derived anti-mouse Ig antibodies that have been conjugated with biotin, a small molecule that can bind to the protein avidin with high affinity. This biotinylation allows the antibodies to be easily detected and visualized in experimental procedures.
Automatically generated - may contain errors
2 protocols using biotinylated goat anti mouse ig 1010 08
1
Sensitive Sandwich ELISA for Mouse Ig
2
Sensitive Sandwich ELISA for Mouse Ig
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A FluoroNunc Maxisorp 96-well plate was coated with 100 µg/mL salmon sperm dsDNA (AM9680, ThermoFisher Scientific) in PBS and incubated overnight at 4 °C. Wells were blocked with 200 µL TBS containing 1% bovine serum albumin (BSA) (A4503, Sigma-Aldrich) for 1 hour at RT and washed thrice with TBS/Tw (TBS containing 0.05% v/v Tween-20 (8.17072.1000, Merck)). Samples, standards, and quality controls were diluted in TBS/Tw containing 5 mM EDTA and 0.1% w/v BSA, and subsequently added in duplicates. The plate incubated at 37 °C for 1 hour. Wells were washed thrice in TBS/Tw and incubated with biotinylated goat-anti-mouse Ig (1010-08, Southern Biotech), 1 µg/ml TBS/Tw, at 37 °C for 1 hour. Wells were washed 3 times in TBS/Tw, and Eu3+-labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 µM EDTA was subsequently added to the wells and incubated at RT for 1 h. Finally, the wells were washed thrice with TBS/Tw, and 200 µL enhancement buffer (AMPQ99800, Amplicon) was added. The plate was shaken for 5 min and counts were read in a time-resolved fluorometry plate reader (Victor X5, PerkinElmer).
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