HFIP was purchased from Aladdin Chemistry Co., Ltd. (Shanghai, China). Choline chloride (ChCl), tetrabutylammonium chloride (TBAC), cetyltrimethylammonium bromide (CTAB), salmon sperm DNA sodium salt, ribonucleic acid (yeast) (RNA) and BSA were purchased from Macklin (Shanghai, China). (NH4)2SO4, K2HPO4, KH2PO4, Na2CO3, Na2HPO4, Na2SO4, MgSO4, hydrochloric acid (HCl), ammonia solution (25–28%, w/v) and ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA-Na2·2H2O) were purchased from Sinopharm Chemical Reagent, Co., Ltd. (Shanghai, China). Tris-hydrochloride (Tris-HCl) was purchased from Guangzhou Saiguo Biotech Co., Ltd (Guangzhou, China). All other chemicals used were of analytical reagent grade and were not further purified unless otherwise indicated. Deionized water (18.25 MΩ) was used in all experiments.
Human whole blood samples from healthy volunteers were provided by the Wuhan Children's Hospital (China). Appropriate amounts of anticoagulant were added to the blood samples which were then stored at −20 °C until use.
Human whole blood samples from healthy volunteers were provided by the Wuhan Children's Hospital (China). Appropriate amounts of anticoagulant were added to the blood samples which were then stored at −20 °C until use.