DOTAP (890890P), DOPE (850725P), DOPC (850375P), and PE-PEG (880120P) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). Tetrachloroauric acid (HAuCl4·3H2O) (520918) and sodium citrate tribasic trihydrate (S4641) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture media RPMI 1640 (10-040-CV) was obtained from Corning Inc. (Corning, NY, USA). FBS (16000-044) and Penn-Strep (15140-122) were purchased from Life Technologies (Grand Island, NY, USA), Opti-MEM was from Thermo Fisher Scientific (Waltham, MA, USA). The scrambled control siRNA (cat. SIC001), siRNAs against human MICU1 (SASI_Hs01_00070249), CBS (SASI_Hs01_00214623), and CAV1 (SASI_Hs01_00199504) were procured from Sigma-Aldrich. The following primary antibodies were purchased from the specified vendor: rabbit monoclonal anti-MICU1 (#12524, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-CBS (#sc-67154, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CAV1 (#SAB871521112, Sigma Aldrich), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich), and anti-Ki67 (no. ab833, Abcam).
Fbs 16000 044
Manufactured by Thermo Fisher Scientific
Sourced in United States
FBS (16000-044) is a high-quality fetal bovine serum (FBS) product offered by Thermo Fisher Scientific. FBS is a common cell culture supplement used to support the growth and proliferation of various cell lines in vitro. The product code 16000-044 represents the specific lot or catalog number of this FBS offering.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem, by Thermo Fisher Scientific (4 mentions)
Sodium citrate tribasic trihydrate s4641, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions)
Rabbit polyclonal anti cbs, by Santa Cruz Biotechnology (1 mentions)
Anti cav1, by Merck Group (1 mentions)
Penn strep, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 10 040 cv, by Corning (1 mentions)
Anti ki67, by Abcam (1 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)
Gold 3 chloride trihydrate 520918, by Merck Group (1 mentions)
Tgf β1 240 b, by R&D Systems (1 mentions)
Neutral protease, by Serva Electrophoresis (1 mentions)
4 protocols using fbs 16000 044
1
Lipid Nanoparticle Synthesis and Characterization
2
Investigating Signaling Pathways in Cell Cultures
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Gold (III) chloride trihydrate (520 918) and sodium citrate tribasic trihydrate (S4641) were bought from Sigma‐Aldrich (St. Louis, MO). Cell culture media RPMI 1640 (10‐040‐CV) was obtained from Corning Inc. (Corning, NY, USA). FBS (16000‐044) and Penn‐Strep (15140‐122) were purchased from Life Technologies (Grand Island, NY, USA), Opti‐MEM was from Thermo Fisher Scientific (Waltham, MA, USA). The following primary antibodies were purchased from the specified vendor: rabbit monoclonal anti‐IGFBP2 (#3922S), ‐PTEN (#9552S), ‐mTOR (#2972S) and ‐AKT S473 (#4058S) (Cell Signaling Technology, Danvers, MA, USA), rabbit anti‐GAPDH (#9545) and ‐tubulin (#T5168) (Sigma Aldrich). Lipofectamine RNAiMAX transfection reagent (13 778 150) was bought from Invitrogen (Waltham, Massachusetts).
3
Cultured Rat Renal Cell Responses to TGFβ1
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The rat proximal tubular epithelial cells (NRK-52E) and the renal interstitial fibroblast cells (NRK-49F) were planted on a petri dish and cultured at 37°C, 5% CO2 environment. The culture medium was DMEM/F12 (12400–024), containing 10% fetal bovine serum (FBS; 16000-044) and 1% penicillin-streptomycin (15140–122) (all from Life Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA). When cells grew to 80% confluency, the culture medium was replaced with serum-free medium for 16 h. After 16 h, the serum-free medium was replaced again, and TGFβ1 (240B; R&D Systems, Inc., Minneapolis, MN, USA) was added to stimulate the cells. Cells and supernatants were collected at different times. The control group was collected in the same way.
4
Isolation of Pancreatic Islets from Tumor Patients
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We obtained pancreatic tissues from non-diabetic patients who underwent pancreatic surgery for tumors [14 (link)]. The study was approved by the Institutional Review Board of Seoul National University Hospital (IRB No. 0901-010-267 and 2110-151-1266). All eight participants (three men, five women) provided written informed consent. The mean±standard deviation of age was 54.5±16.0 years, body mass index 25.6±3.6 kg/m2, and glycosylated hemoglobin 5.7%±0.3% (Supplementary Table 1 ).
We isolated islets from 0.80 g (median range, 0.21 to 5.0) of pancreatic tissues that were grossly normal. In less than 1.5 hours stored in histidine-tryptophan-ketoglutarate solution, the tissues were chopped and digested with collagenase NB and neutral protease (SERVA Electrophoresis GmbH, Heidelberg, Germany). Subsequently islets were separated using Ficoll gradients (P04-69600, Pan-biotech, Aidenbach, Germany). After washing the islets with Hanks’ balanced salt solution (HBSS; LB 003-02, WELGENE, Gyeongsan, Korea) containing 10% fetal bovine serum (FBS; 16000-044, ThermoFisher, Waltham, MA, USA), we hand-picked islets and incubated overnight before experiments, as described before [9 (link)]. The isolation yield was about 700 islets/g pancreas (Supplementary Table 1 ).
We isolated islets from 0.80 g (median range, 0.21 to 5.0) of pancreatic tissues that were grossly normal. In less than 1.5 hours stored in histidine-tryptophan-ketoglutarate solution, the tissues were chopped and digested with collagenase NB and neutral protease (SERVA Electrophoresis GmbH, Heidelberg, Germany). Subsequently islets were separated using Ficoll gradients (P04-69600, Pan-biotech, Aidenbach, Germany). After washing the islets with Hanks’ balanced salt solution (HBSS; LB 003-02, WELGENE, Gyeongsan, Korea) containing 10% fetal bovine serum (FBS; 16000-044, ThermoFisher, Waltham, MA, USA), we hand-picked islets and incubated overnight before experiments, as described before [9 (link)]. The isolation yield was about 700 islets/g pancreas (
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