Mouse monoclonal anti-actin (clone C4), rabbit polyclonal anti-p-FAK (Y397) and mouse monoclonal anti-FAK (Clone 4.47) were purchased from Millipore (Billerica, MA, USA), rabbit polyclonal anti-SCP-2 was from Abcam (Cambridge, UK) and rabbit polyclonal anti-p-FAK (Y925) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-p-Paxillin (Y118), anti-Paxillin, anti-Akt (S473) and anti-Akt were purchased from Cell signaling (MA, USA). Human full length His-tagged ADRM1 (His-ADRM1) and GST-tagged HR23B (GST-HR23B) proteins were purchased from ATgen (Gyeonggi-do, Korea) and Abnova (Taipei, Taiwan) respectively.
Mouse monoclonal anti actin clone c4
Manufactured by Merck Group
Sourced in United States
Mouse monoclonal anti-actin (clone C4) is a laboratory reagent used to detect and quantify the presence of the actin protein in biological samples. It functions as a specific antibody that binds to actin, allowing for its identification and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti akts473, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti human cd147 antibody clone mem m6 1, by Novus Biologicals (1 mentions)
Alphalisa reagents, by PerkinElmer (1 mentions)
Alphalisa universal buffer, by PerkinElmer (1 mentions)
Alphascreen streptavidin coated donor beads, by PerkinElmer (1 mentions)
Alphalisa unconjugated acceptor beads, by PerkinElmer (1 mentions)
Enspire alpha 2300 multilabel plate reader, by PerkinElmer (1 mentions)
2 protocols using mouse monoclonal anti actin clone c4
1
Antibody-based protein detection protocol
2
Exosome biomarker detection protocol
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The following antibodies were used for immunoblotting: mouse monoclonal anti-human CD63 antibody (clone H5C6, dilution 1:200) from BD Biosciences, mouse monoclonal anti-human CD9 antibody (clone ALB 6, dilution 1:200) from SantaCruz Biotechnology, mouse monoclonal anti-human CD147 antibody (clone MEM-M6/1, dilution 1:1,000) from Novus Biologicals and mouse monoclonal anti-Actin (clone C4, dilution 1:1,000) from Millipore. The secondary antibody (horseradish peroxidase-labeled sheep anti-mouse) were purchased from GE HealthCare.
The following antibodies used for ExoScreen and ELISA were developed in Shionogi & Co., LTD.: mouse monoclonal anti-human CD63 antibody (clone 8A12) and mouse monoclonal anti-human CD9 antibody (clone 12A12). Mouse monoclonal anti-human CD147 antibody (clone MEM-M6/1) was purchased from Novus Biologicals. Antibodies were used to modify either acceptor bead or biotin following the manufacturer’s protocol.
AlphaLISA reagents (Perkin Elmer, Inc., Waltham, MA 02451, USA) consisted of AlphaScreen Streptavidin-coated donor beads (6760002), AlphaLISA Unconjugated-acceptor beads (6062011) and AlphaLISA Universal buffer (AL001F). AlphaLISA assays were performed in 96-well half-area white plates (6005560) and read in an EnSpire Alpha 2300 Multilabel Plate reader (Perkin Elmer, Inc.).
The following antibodies used for ExoScreen and ELISA were developed in Shionogi & Co., LTD.: mouse monoclonal anti-human CD63 antibody (clone 8A12) and mouse monoclonal anti-human CD9 antibody (clone 12A12). Mouse monoclonal anti-human CD147 antibody (clone MEM-M6/1) was purchased from Novus Biologicals. Antibodies were used to modify either acceptor bead or biotin following the manufacturer’s protocol.
AlphaLISA reagents (Perkin Elmer, Inc., Waltham, MA 02451, USA) consisted of AlphaScreen Streptavidin-coated donor beads (6760002), AlphaLISA Unconjugated-acceptor beads (6062011) and AlphaLISA Universal buffer (AL001F). AlphaLISA assays were performed in 96-well half-area white plates (6005560) and read in an EnSpire Alpha 2300 Multilabel Plate reader (Perkin Elmer, Inc.).
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