Anti α tubulin ab52866

Manufactured by Abcam

Sourced in United Kingdom

Anti-α-Tubulin (ab52866) is a primary antibody that specifically recognizes the alpha-tubulin protein. Alpha-tubulin is a key component of microtubules, which are essential cytoskeletal structures involved in various cellular processes such as cell division, intracellular transport, and cell motility. This antibody can be used for the detection and localization of alpha-tubulin in a variety of applications, including immunocytochemistry, immunohistochemistry, and Western blotting.

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Lab products found in correlation

Ab2787, by Abcam (1 mentions) Ab195977, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) P irf3 ser396, by Cell Signaling Technology (1 mentions) P tbk1 ser172, by Cell Signaling Technology (1 mentions) P nf κb p65, by Cell Signaling Technology (1 mentions) Anti myc ta150121, by OriGene (1 mentions) Anti β actin ab3280, by Abcam (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Ritonavir, by Merck Group (1 mentions) Nocodazole, by Merck Group (1 mentions) Anti gfap z0334, by Agilent Technologies (1 mentions) Blebbistatin, by Merck Group (1 mentions) Latrunculin a, by Santa Cruz Biotechnology (1 mentions) Jasplakinolide, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

α-tubulin (Ab52866) Anti-α-tubulin Ab52866 α-tubulin Anti-tubulin

3 protocols using anti α tubulin ab52866

Western Blot Analysis of Insect Proteins

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Modified western blot analyses were performed according to previously described methods (Zhai et al., 2013 (link)). The proteins were separated on a 12% SDS-PAGE gel and transferred to PVDF membranes (0.4 μm, Millipore), before the membranes were immunoblotted using the following antibodies: anti-FoxO (Forkhead box protein O, ab195977), anti-Hsp70 (Heat shock 70 kDa protein, ab2787), and anti-α-Tubulin (ab52866) from Abcam. Anti-FKBP12 (FK506-binding protein 12), anti-JHAMT (Juvenile hormone acid O-methyltransferase), and anti-YP1 (yolk protein 1) polyclonal sera were prepared in our laboratory. IgG goat anti-rabbit and anti-mouse antibodies conjugated with HRP were used as secondary antibodies (1:5,000, Abcam, United Kingdom), and the membranes were visualized by ECL (enhanced chemiluminescence). Three biological replicates were performed for each protein.

Zhai Y., Dong X., Gao H., Chen H., Yang P., Li P., Yin Z., Zheng L, & Yu Y. (2019). Quantitative Proteomic and Transcriptomic Analyses of Metabolic Regulation of Adult Reproductive Diapause in Drosophila suzukii (Diptera: Drosophilidae) Females. Frontiers in Physiology, 10, 344.

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Signaling Pathway Antibody Protocol

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Antibodies specific for p-IKKε (Ser172) (#8766), IKKε (#3416), p-IRF3 (Ser396) (#4947), p-NF-κB p65 (#3033), NF-κB p65 (#8242), p-TBK1 (Ser172) (#5483), TBK1 (#3504), p-ERK (#4370), ERK (#4695), p-JNK (#4668), JNK (#9285), p-p38 (#9215), p38 (#8690), and MAVS (#4983) and HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-IRF3 (sc-15991) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-DDK (Flag) (TA50011-1), and anti-Myc (TA150121) were obtained from Origene (Rockville, MD, USA). Anti-TAOK1 (ab92601), anti-DYNC (ab23905), anti-α-tubulin (ab52866), and anti-β-actin (ab3280) were purchased from Abcam Biotechnology (Cambridge, UK). Anti-HA (51064-2-AP), anti-DDX58 (RIG-I) (20566-1-AP), and anti-MAP4 (11229-1-AP) were purchased from Proteintech (Wuhan, Hubei, China). Anti-p-MAP4 (Ser696) (#PA5-64526) was purchased from Invitrogen (Thermo Fisher Scientific, Scotts Valley, CA, USA).

Luo X., Ji R., Liu Q., Xiao X., Song W., An H., Li Y, & Zhou J. (2023). Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis. Journal of Innate Immunity, 15(1), 380-396.

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Antibodies and Cytoskeleton Modulators

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Restriction enzymes and buffers were from Promega. Anti-vimentin antibodies were: mouse monoclonal V9 clone (sc-6260) and its Alexa-488 conjugate from Santa Cruz Biotechnology, and mouse anti-vimentin monoclonal antibody (V5255) from Sigma. Anti-actin (A2066) and anti-Hsp70 (H5147) were from Sigma, and anti-α-tubulin (ab52866) and anti-desmin (ab15200-1) from Abcam. Anti-GFAP (Z0334) was from Dako. C3 transferase toxin was from Cytoskeleton. Latrunculin A and jasplakinolide were from Santa Cruz Biotechnology. HNE and PGA₁ were from Cayman Chemical. 4,6-Diamidino-2-phenylindole (DAPI), cytochalasin B, nocodazole, blebbistatin, and ritonavir were from Sigma.

Duarte S., Viedma-Poyatos Á., Navarro-Carrasco E., Martínez A.E., Pajares M.A, & Pérez-Sala D. (2019). Vimentin filaments interact with the actin cortex in mitosis allowing normal cell division. Nature Communications, 10, 4200.

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