Jem 1400 flash tem microscope

Mice were perfused with 4% PFA. First, the striata were acquired quickly after the brain samples were removed from the skulls and fixed in 2.5% glutaraldehyde at 4°C for 2 h. Then, the samples were washed four times in PBS. After that, they were postfixed in 1% osmic acid at 4°C for 2 h and then incubated in the 2% uranyl acetate aqueous solution for next 2 h. Following dehydration in a conventional acetone gradient from 50 to 100% at 4°C for 15 min, the samples were placed in a mixture of 100% dehydrating agent and the same amount of Epon 812 embedding agent at RT for 2 h. Subsequently, the samples were soaked in pure embedding agent at RT overnight. When the embedding agent had solidified, the blocks were cut into 50‐ to 80‐nm ultrathin sections. After staining with uranyl acetate and lead citrate, the ultrathin sections were examined under a JEOL JEM‐1400 Flash TEM microscope. The length of mitochondria was measured by the NIH ImageJ software.