CSR (purity > 99%, determined by HPLC) was purchased from Extrasynthese (Genay Cedex, France). Indomethacin, crystal violet, phosphate‐buffered saline (PBS) and dimethylsulfoxide (DMSO) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). CCK-8 (Cell Counting Kit-8) was purchased from TransGen Biotech (Beijing, China). Recombinant human IL-6 and recombinant human soluble IL-6 receptor α (sIL-6R) were purchased from PeproTech (Rocky Hill, NY, USA). Janus kinase 2 (JAK2, ab39636) and phospho-JAK2 (Tyr1007/1008, ab32101) monoclonal antibodies, HRP-conjugated secondary antibodies (ab7090, ab97040), and Annexin V-FITC Apoptosis Staining / Detection Kit (#ab14085) were purchased from Abcam (Cambridge, CB2 0AX, UK). B-cell lymphoma 2 (Bcl-2, sc-7382) and GAPDH (sc-365062) monoclonal antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Myeloid cell leukemia 1 (Mcl-1, #94296), caspase-9 (#9508), caspase-3 (#9662), STAT3 (#12640), phospho-STAT3 (Tyr705, #9131) and lamin B1 (#12586) monoclonal antibodies were obtained from Cell Signaling Technology (Boston, MA, USA). All other chemicals used were purchased from Sigma and were of analytical grade.
Annexin 5 fitc apoptosis staining detection kit ab14085
Manufactured by Abcam
Sourced in United Kingdom
The Annexin V-FITC Apoptosis Staining / Detection Kit (#ab14085) is a tool for detecting and quantifying apoptosis in cell cultures. The kit contains Annexin V-FITC, a fluorescent dye that binds to phosphatidylserine, a protein that is exposed on the surface of apoptotic cells. This allows for the identification and enumeration of cells undergoing apoptosis using techniques such as flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Merck Group (1 mentions)
Recombinant human il 6, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Lamin b1 12586, by Cell Signaling Technology (1 mentions)
Gapdh sc 365062, by Santa Cruz Biotechnology (1 mentions)
Ab39636, by Abcam (1 mentions)
Goat anti mouse igg pe sc 3738, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 9 9502, by Cell Signaling Technology (1 mentions)
Anti caspase3 9662, by Cell Signaling Technology (1 mentions)
2 protocols using annexin 5 fitc apoptosis staining detection kit ab14085
1
Indomethacin Inhibits IL-6-Induced Signaling Pathways
2
Apoptosis Pathway Analysis Protocol
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Anti-p53 (sc-126), anti-PTEN (sc-7974), goat anti-mouse IgG-FITC (sc-2010), anti-β-Actin (sc-47778), anti-Bcl2 (sc-509), anti-BAX (sc-20067), anti-Cytochrome c (sc-13156), anti-XIAP (sc-55551) and goat antimouse IgG-PE (sc-3738) were from SANTA CRUZ BIOTECHNOLOGY, INC., Santa Cruz, California, USA.
Anti-AKT phospho S473 (#9271), anti-Caspase 3 (#9662) and anti-Caspase 9 (#9502) were from Cell Signaling Technology, Danvers, Massachusetts, USA; DAPI (D9542), Propidium iodide (P4170), RIPA buffer, polyvinyl di uoride (PVDF) membranes (IPVH00010) and anti-AKT (SAB4500800) was from Sigma (St Louis, MO, USA). Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085) was purchased from Abcam, Cambridge, UK.
Anti-AKT phospho S473 (#9271), anti-Caspase 3 (#9662) and anti-Caspase 9 (#9502) were from Cell Signaling Technology, Danvers, Massachusetts, USA; DAPI (D9542), Propidium iodide (P4170), RIPA buffer, polyvinyl di uoride (PVDF) membranes (IPVH00010) and anti-AKT (SAB4500800) was from Sigma (St Louis, MO, USA). Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085) was purchased from Abcam, Cambridge, UK.
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