Lentiviral shRNA (ATG7 and BECLIN1) constructs were purchased from Open Biosystems (RMM4534_019584 and RMM4534_028835) (Ottawa, ON, Canada). ATG7 and BECLIN1 shRNAs were transfected into PC3, LNCaP, or MCF7 cells either alone or together with packaging plasmids following the manufacturer's protocol (Invitrogen ViraPower Lentiviral Expression Systems kit, Carlsbad, CA). Cells were passaged and plated in a six-well plate and allowed to adhere for 24 hours before infection. The silencing efficacy of the various shRNAs was assessed by Western blot analysis of ATG7 and BECLIN1 proteins using polyclonal antibodies (Sigma-Aldrich, Oakville, ON, Canada).
Rmm4534 019584
Manufactured by Thermo Fisher Scientific
Sourced in Canada
The RMM4534_019584 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to perform basic laboratory tasks. However, a detailed and unbiased description of its specific features and capabilities is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rmm4534 019584
1
Silencing ATG7 and BECLIN1 in Cancer Cells
2
Knockdown of ATG7 and BECLIN1 in PC3 Cells
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The PC3 cells with knockdown of ATG7 and BECLIN1 (or both) were generated. Lentiviral shRNA (ATG7 and BECLIN1) constructs were purchased from Open Biosystems (RMM4534_019584 and RMM4534_028835). The ATG7 and BECLIN1 shRNAs were transfected into PC3 cells, either alone or together with packaging plasmids following the manufacturer's protocol (Invitrogen ViraPower Lentiviral Expression Systems kit, Carlsbad, CA, USA). The silencing efficacy of the various shRNA was assessed by WB analysis of ATG7 and BECLIN1 proteins using polyclonal antibodies.
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