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Anti st2

Manufactured by R&D Systems
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Anti-ST2 is a laboratory reagent used for the detection and quantification of the ST2 protein. ST2 is a member of the interleukin-1 receptor family and plays a role in various biological processes. This product can be used in research applications to study the expression and function of ST2.

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Lab products found in correlation

Il 33, by R&D Systems (2 mentions) Human recombinant murine il 33, by R&D Systems (1 mentions)

Spelling variants of this product

Anti-ST2 antibody (6 citations) Anti-human ST2 antibody (3 citations)

2 protocols using anti st2

1

Macrophage Migration Assay with IL33

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In vitro migration assay was performed in a Transwell system with a polycarbonate membrane of 6.5-mm diameter with a 3-μm pore size as described previously(29 (link), 30 (link)). CD3−CD19−CD14+ macrophages were isolated and sorted from normal blood and fresh colon cancer tissues, and were subjected to the Transwell migration assay. Macrophages were added to the top chamber. IL33 (0–100 ng/mL, R&D Systems) or anti-ST2 (50 ng/mL, R&D Systems) was added to the bottom chamber. After an 8-hour incubation at 37°C, the phenotype and number of migrated cells in the bottom chambers were determined by FACS.
Fang M., Li Y., Huang K., Qi S., Zhang J., Zgodzinski W., Majewski M., Wallner G., Gozdz S., Macek P., Kowalik A., Pasiarski M., Grywalska E., Vatan L., Nagarsheth N., Li W., Zhao L., Kryczek I., Wang G., Wang Z., Zou W, & Wang L. (2017). IL33 Promotes Colon Cancer Cell Stemness via JNK Activation and Macrophage Recruitment. Cancer research, 77(10), 2735-2745.
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2

Cell Proliferation Assay with TMZ, IL-33, and Anti-ST2

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Cell proliferation was evaluated using the Cell counting kit-8 (CCK-8) assay according to the protocol. Briefly, cells were seeded in 96-well plates and incubated with various concentrations of TMZ (Selleck), IL-33 (Human recombinant murine IL-33, R&D Systems) or Anti-ST2 (Human ST2/IL-33R Antibody, R&D Systems). Subsequently, CCK-8 solution was added, and the viable cells were quantified using IMARK microplate reader at 450 nm of absorbance. Each experiment was performed in triplicate and repeated for three times.
Lin L., Li Y., Liu M., Li Q., Liu Q, & Li R. (2020). The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation. Aging (Albany NY), 12(2), 1685-1703.
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