Griess reaction system

Manufactured by Promega

Sourced in United States

The Griess reaction system is a colorimetric assay used to detect and quantify nitrite (NO2-) in various sample types. The system involves the reaction of nitrite with sulfanilamide and N-(1-naphthyl)ethylenediamine dihydrochloride to produce a purple azo dye that can be measured spectrophotometrically.

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Lab products found in correlation

β hydroxybutyrate assay kit, by Merck Group (1 mentions) Wallac envision microplate reader, by PerkinElmer (1 mentions) Elisa max standard set human il 6, by BioLegend (1 mentions) Tnf alpha human uncoated elisa kit, by Thermo Fisher Scientific (1 mentions) Plex mouse procartaplex panel, by Thermo Fisher Scientific (1 mentions)

3 protocols using griess reaction system

Blood Metabolite Quantification

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Collected by cardiocentesis or retro orbital puncture, PH mouse blood was centrifuged
(4°C, 3,000 rpm, 10 min) and serum was deproteinized using 30,000 MWCO (for nitrite assay)
or 10,000 MWCO (for β-hydroxybutyrate assay) filter membrane (Sartorius, Göttingen,
Germany). Serum nitrite concentration was measured with Griess Reaction System (Promega,
WI, USA) and β-hydroxybutyrate was measured with β-hydroxybutyrate assay kit
(Sigma-Aldrich, MO, USA).

Yu Y., Tamai M, & Tagawa Y.I. (2017). Nitric oxide is critical for avoiding hepatic lipid overloading via IL-6 induction during liver regeneration after partial hepatectomy in mice. Experimental Animals, 66(4), 293-302.

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Nitrite Assay for Measuring NO Production

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The nitrite concentration in the culture medium was measured as an indicator of NO production by the Griess reaction system (Promega, WI, USA). RAW 264.7 cells (5 × 10⁴ cells/well) in 96-well plates were cultured for 24 h. The cells were then treated with the samples list above plus LPS (1 μg/mL) or LPS alone for 20 h. The supernatant was mixed with the same volume of Griess reagent and incubated at room temperature for 5 min. The concentration of nitrite was determined by measuring the absorbance with a Wallac EnVision™ Microplate Reader (PerkinElmer).

Chun J.M., Nho K.J., Kim H.S., Lee A.Y., Moon B.C, & Kim H.K. (2014). An ethyl acetate fraction derived from Houttuynia cordata extract inhibits the production of inflammatory markers by suppressing NF-кB and MAPK activation in lipopolysaccharide-stimulated RAW 264.7 macrophages. BMC Complementary and Alternative Medicine, 14, 234.

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Cytokine and Nitric Oxide Measurement

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NO produced by macrophages was measured using the Griess reaction system (Promega). Mouse IL-6, IL-1β, TNF-α, IFN-γ and GM-CSF level in the cultured medium was determined by Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel according to the manufacturer’s recommendations (Invitrogen). Human IL-6 and TNF-α in culture medium was measured by ELISA MAX Standard Set Human IL-6 (BioLegend, USA) and TNF alpha Human Uncoated ELISA Kit (Invitrogen, USA), respectively, following manufacturer’s instructions.

Zhang R., Cheung C.Y., Seo S.U., Liu H., Pardeshi L., Wong K.H., Chow L.M., Chau M.P., Wang Y., Lee A.R., Kwon W.Y., Chen S., Chan B.K., Wong K., Choy R.K, & Ko B.C. (2021). RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation. Frontiers in Immunology, 12, 679184.

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