Reagents for real time pcr

Mouse E-cadherin (#610182) and N-cadherin (#610920) antibodies were from BD Transduction Laboratories; anti–β-actin was from Sigma, anti-vimentin (ab11256) was from abCam. SMAD1/2/3 (Sc-7960), SNAI 1 (Sc-28199), RhoA (Sc-418), c-Myc (Sc-789) antibodies were from Santa Cruz. Alexa488–, Alexa568–, and Alexa647–conjugated secondary antibodies and rhodamine- and Alexa568-conjugated phalloidin were from Molecular Probes. Reagents for real-time PCR were from Applied Biosystems; the RNA isolation kit was from Qiagen. Cytochalasin D and Jasplakinolide were from calbiochem (Millipore). RhoA plasmids were a kind gift from Nathalie Lamarche (McGill University). RhoA beads, MLB lysis buffer and Rac1 mAb were from Millipore (Upstate).

Polyclonal Anti-Feline Infectious Peritonitis Virus (FIPV), 79-1146 (antiserum, Guinea Pig) (Cat # NR-2518) and Alphacoronavirus 1, 79-1146, formerly Feline Infectious Peritonitis Virus (FIPV) (Cat # 43287) were from BEI RESOURCES. CRFK cells were from the laboratory of Dr Yvonne Drechsler (Western University of Health Sciences). The CRFK cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and penicillin–streptomycin (100 μg/ml) in a CO₂ incubator. Anti-FIP N mouse monoclonal antibody (Cat # SM1870P) was from Origene. Anti-guineapig (Cat #: 926-68077) and anti-mouse (Cat #: 926-68072) secondary antibodies were from LI-COR. The PCR reagents were from GenScript. The reagents for real-time PCR were from Applied Biosystems. All other reagents were from Sigma. HisTrap columns were from Cytiva (Cat # 17528601), High Precision Streptavidin (SAX) biosensors were from ForteBio (Cat # 18-0037) and Rosetta(DE3) Competent Cells were from Millipore Sigma (Cat # 70954)..