Aspartate aminotransferase ast assay kit

G2111 was synthesized in our laboratory. Human Serum Albumin (HSA) (20%) was purchased from CSL Behring AG (Philadelphia, PA, USA). N-Succinimidyl 4-(maleimidomethyl) cyclohexane-1-carboxylate (SMCC) was obtained from Beijing Innochem Science & Technology Co., Ltd (Beijing, China). A6 peptide was provided by GUOTAI Biotechnology (Beijing, China). Coumarin 6 and IR780 were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Antibodies against CDK4 and β-actin were obtained from Abcam (Cambridge, MA, USA). Cell Counting Kit-8 (CCK-8) was from Shanghai Epizyme Biomedical Technology Co., Ltd (Shanghai, China). The commercial assay kit for Annexin V-FITC apoptosis detection was acquired from Bestbio (Shanghai, China). Aspartate aminotransferase (AST) assay kit and Alanine aminotransferase (ALT) assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Con A was bought from Solarbio Science & Technology Co., Ltd. (Beijing, China, catalog number: C8110). Malondialdehyde (MDA) assay kit (TBA method), Alanine aminotransferase (ALT) Assay Kit, and Aspartate aminotransferase (AST) Assay Kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China, catalog number: A003-1-2, C009-2-1 and C010-2-1). Total Nitric Oxide (NO) Assay Kit was gotten from Beyotime Biotechnology (Shanghai, China, catalog number: S0021S). Chloral hydrate, UNlQ-10 Column Total RNA Isolation Kit and 2X SYBR Abstart Master Mix was purchased from Sangon Biotech Co., Ltd. (Shanghai, China, catalog number: A600288, B511321 and B110032). The Total Protein Extraction Kit (BOSTER Biological & Technology Co. Ltd., China, catalog number: AR0146), SDS–polyacrylamide gel electrophoresis (SDS-PAGE, Beyotime Biological Technology Co. Ltd., China, catalog number: P0012A), PVDF membranes (Millipore, USA, catalog number: R1CB73920), High-sig ECL Western Blotting Substrate (Tanon Science & Technology Co., Ltd., China, catalog number:180-501), Anti-p38 Rabbit pab, Mapk3k13 Rabbit pab, HRP-conjugated Goat Anti-Rabbit IgG (Additional file 1: Table S1) were used to detect the expression of target protein.

The blood samples of mice from each time point were collected and prepared for serum and anticoagulant blood. Serum hepatic enzyme activities were determined using alanine aminotransferase (ALT) assay kit and aspartate aminotransferase (AST) assay kit (Jiancheng, Nanjing, China), respectively. The levels of white blood cells (WBC), lymphocytes (LYM), granulocytes (GRAN), monocytes (MONO), red blood cells (RBC), hematocrit (HCT), hemoglobin (HGB) and platelet (PLT) were determined using an automatic blood analyzer (Servicebio, Wuhan, China).

Arsenic trioxide (ATO; As₂O₃) was purchased from Beijing Chemical Reagent Corp. (Beijing, China). ATO was dissolved in 1 N NaOH at 0.1 M as a stock solution. LU was obtained from JF-Natural (Tianjin, China). GSH, superoxide dismutase (SOD), total antioxidative capacity (T-AOC), malondialdehyde (MDA), bicinchoninic acid protein, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals were of analytical grade and were obtained commercially.

CTS (Lot, 10321A; viscosity, >200 mP/s; degree of deacetylation, 96.2%) was purchased from Shandong Aokang Biotech Ltd. (Shandong, China). COSI (Lot, HL130813G; M_N≤1,000 g.mol⁻¹; degree of deacetylation, 95.6%) and COSIII (Lot, HL130921G; M_N≤3,000 g.mol⁻¹; degree of deacetylation, 91.1%) were obtained from Shandong Laizhou Highly Bio-Products Co., Ltd. (Shandong, China). Simvastatin tablets were supplied from Merck Sharp & Dohme Pharmaceutical Company Limited (Hangzhou, Zhejiang, China). Triacylglycerol (TG), total cholesterol (TC), HDL cholesterol (HDL-C), LDL-C, and total bile acid (TBA) kits were purchased from BioSino Bio-technology and Science Inc. (Beijing, China). Superoxide dismutase (SOD), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) assay kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other reagents and solvents were of analytical grade.

GL (≥98% purity) and GA (≥98% purity) were acquired from Yuanye Bio-Technology Co. (Shanghai, China). Acetonitrile and methanol were provided by Merck KGaA (Darmstadt, Germany). Formic acid (FA) was obtained from ACS (Wilmington, DE, USA), and CCl₄ and olive oil were bought from Macklin Biochemical Technology Co. (Shanghai, China). Trypsin (1:250) and pepsin (1:10,000) were purchased from Solebo Technology Co. (Beijing, China). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) assay kits were obtained from Jiancheng Bioengineering Institute (Nanjing, China).