Ab38171

Manufactured by Abcam

Ab38171 is a monoclonal antibody that recognizes the protein GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase). GAPDH is a key enzyme involved in glycolysis, the metabolic pathway that converts glucose into energy. This antibody can be used to detect and quantify GAPDH protein levels in various biological samples.

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Lab products found in correlation

Lsm 880 confocal microscope, by Zeiss (2 mentions) Ab84036, by Abcam (1 mentions) Goat anti rabbit af488, by Thermo Fisher Scientific (1 mentions) Alexa 568 anti mouse, by Thermo Fisher Scientific (1 mentions) Pha e, by Merck Group (1 mentions) Bis sulfosuccinimidyl suberate bs3, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab38171

Cellular Uptake Experiments with TZM-AF700 and Tf-AF568

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For uptake experiments, cells were plated on 8 well µ-slides (Ibidi, Fitchburg, WI, USA) at 50,000 cells per well and cultured overnight followed by 24 h incubation with TZM-AF700 and Tf-AF568 (Thermo Fisher) in cell culture medium. Cells were fixed for 15 min with 4% paraformaldehyde (PFA) and processed for immunocytochemistry using rabbit monoclonal HER2 antibody (Cell Signaling cat#2165, 1:500), rat HER2-AF488 (Bio Rad MCA1788A488 1:50), rabbit monoclonal EEA1 antibody (Cell Signaling cat#3288, 1:500), mouse monoclonal EEA1 (BD Biosciences cat#610457 1:500), mouse monoclonal TfR antibody (Abcam ab38171, 1:500), or rabbit polyclonal TfR antibody (Abcam ab84036, 1:500). Secondary antibodies F(ab) goat anti-mouse AF647, AF568, goat anti rabbit AF488, AF555, AF647 (Thermo Fisher Scientific) were used at 1:500 dilution. Samples were imaged on Zeiss LSM 880 confocal microscope using identical settings across all samples.

Rudkouskaya A., Smith J.T., Intes X, & Barroso M. (2020). Quantification of Trastuzumab–HER2 Engagement In Vitro and In Vivo. Molecules, 25(24), 5976.

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Erythroagglutinating Lectin-Mediated RBC Analysis

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Human O+ red blood cells (RBCs) were provided by the Australian Red Cross Blood service. Erythroagglutinating lectin from red kidney bean (Phaseolus vulgaris; phytohemagglutinin (PHA-E)) was obtained as lyophilized powder from Sigma Aldrich and dissolved in PBS. Glutaraldehyde (Grade I, 25% in H₂O), (3-Aminopropyl)triethoxysilane (APTES), EPB41 rabbit antibody (HPA028414) and DMTN rabbit antibody (HPA024290) were also purchased from Sigma-Aldrich. Bis(sulfosuccinimidyl)suberate (BS3), Alexa 488 anti-mouse/rabbit, Alexa 568 anti-mouse, Alexa 647 anti-mouse/rabbit, Ankyrin 1 mouse antibody (S388A-10) and FITC-conjugated TfR/CD71 monoclonal antibody (MEM-75, MA1-19577) were obtained from Life Technologies (Thermo Fisher). Anti-CR1/CD35 (ab25, ab76520) and anti-TfR/CD71 (ab38171) antibodies were obtained from Abcam. The pre-conjugated antibody to CD71 was used in shearing experiments to prevent aggregate formation when using secondary labels.

Blanch A.J., Nunez-Iglesias J., Namvar A., Menant S., Looker O., Rajagopal V., Tham W.H., Tilley L, & Dixon M.W. (2021). Multimodal imaging reveals membrane skeleton reorganisation during reticulocyte maturation and differences in dimple and rim regions of mature erythrocytes. Journal of Structural Biology: X, 6, 100056.

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