C57BL/6 J male mice (10–12 weeks old) were given a single injection of either AC (100 mg kg−1) or vehicle and the cerebellum, hippocampus, and hypothalamus collected 2 h (N = 5 per group) or 24 h (N = 5 per group) postinjection and stored in RNAlater tissue storage solution (Life Technologies, UK) at < 4 °C until processing. Total RNA was isolated from the brain regions and reverse transcribed, with cycle threshold (Ct) values generated as described previously (Abulkassim et al. 2016 (link)). qRT-PCR results were analysed using the relative quantification method of comparative Ct (ΔΔCt), with β-actin acting as the calibrator gene. All primer sequences and RT-PCR methods were as previously described (Abulkassim et al. 2016 (link)), with reactions run in triplicate.
Rnalater tissue storage solution
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
RNAlater tissue storage solution is a reagent that immediately stabilizes and protects cellular RNA in fresh tissue samples. It preserves the RNA expression profile of the tissue, preventing degradation during sample collection, handling, and storage.
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Lab products found in correlation
2 protocols using rnalater tissue storage solution
1
Assessing gene expression changes in mouse brain regions following acute drug exposure
2
LPS-Induced Neuroinflammation in Mice
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C57BL/6J male mice (10-12 weeks old) were given a single injection of either LPS (1 mg kg - 1 in PBS) or PBS alone and the cerebellum, hippocampus and hypothalamus collected either 2 hours (PBS n=5, LPS n=4) or 24 hours (n = 5 per group) post injection and stored in RNAlater tissue storage solution (Life Technologies, Paisley, UK) at <4 o C until processing.
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