Enhanced chemiluminescence ecl western blot detection system

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA). Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). Phospho-c-Src (Cat# 6943), phospho-Jak2 (Cat# 3776), phospho-ERK1/2 (Cat# 4370), phospho-STAT3 (Cat# 9145), COX-2 antibody (Cat# 12,282) antibodies were from Cell Signaling (Danver, MA). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Cat# GTX627408) antibody was from GeneTex (Irvine, CA, USA). Celecoxib (CLC), AG490, U0126, cucurbitacin E (CBE), Sc-19220, L798-106, and GW627368 were from Santa Cruz (Santa Cruz, CA). PP1 was from Biomol (Plymouth Meeting, PA). Bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL). Bradykinin (BK), enzymes, and other chemicals were from Sigma (St. Louis, MO).

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA, USA). Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). PhosphoPlus ERK1/2 (Thr²⁰²/Tyr²⁰⁴) antibody (Cat. #4370) was from Cell Signaling (Danvers, MA, USA). ERK2 antibody (Cat. #sc-154) was from Santa Cruz (Santa Cruz, CA, USA). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat. #4699–9555) was from Biogenesis (Bournemouth, UK). Glial fibrillary acidic protein (GFAP) antibody (Cat. #Z0334) was from DAKO (Carpinteria, CA, USA). Neuron-specific enolase (NSE) (Cat. #ab79757) and Neuronal nuclear protein (NeuN) (Cat. #ab177487) antibodies were from Abcam (Waltham, MA, USA). D-Arg-[Hyp³, Thi⁵, D-Tic⁷, Oic⁸]-BK (Hoe140), GM6001, rottlerin, and PD98059 were from Biomol (Plymouth Meeting, PA, USA). The bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL, USA). Bradykinin (BK), phorbol 12-myristate 13-acetate (PMA), enzymes, and other chemicals were from Sigma (St. Louis, MO, USA).

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA, USA). The Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). The phospho-ERK1/2 (Thr202/Tyr204) (Cat.#4370), phospho-p38 (Thr180/Tyr182) (Cat.#4511), phospho-JNK1/2 (Thr183/Tyr185) (Cat.#4668), and phospho-p65 NF-κB (Ser536) (Cat.#3033) antibodies were from Cell Signaling (Danver, MA, USA). The anti-ZO-1 (Cat.#sc-33725) antibody was from Santa Cruz (Dallas, TX, USA). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA, USA). The R-7050, U0126, SB202190, SP600125, Bay11-7082, and MMP2/9 inhibitor (2/9i) were from Enzo (Farmingdale, NY, USA). The bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL, USA). The tumor necrosis factor-α (TNF-α) was from R&D Systems (Minneapolis, MN, USA). The sophoraflavanone G (SG) was from ChemFaces (Wuhan, China). The enzymes and other chemicals were from Sigma (St. Louis, MO, USA).